Jarolim E, Matthiesen F, Skov P S, Tejkl M, Oesterballe O, Poulsen L K
Medical Department TTA, State University Hospital, Copenhagen, Denmark.
Int Arch Allergy Appl Immunol. 1990;92(3):254-64. doi: 10.1159/000235186.
Patients allergic to grass pollen were hyposensitized with two major allergenic components or whole extract of timothy grass pollen. Specific IgE, IgG1, and IgG4 formed during immunotherapy were analyzed by immunoblotting. Similar antibody-binding patterns were observed in both patient groups. Inhibition experiments using allergenic components isolated by preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that all antigenic components of timothy grass pollen detected in immunoblot dispose of private and cross-reactive determinants for binding of human IgE. The worse clinical outcome of immunotherapy after hyposensitization with two cross-reactive components did, therefore, not correlate with a specific antibody formation pattern.
对禾本科花粉过敏的患者用梯牧草花粉的两种主要变应原成分或全提取物进行了减敏治疗。通过免疫印迹法分析了免疫治疗期间形成的特异性IgE、IgG1和IgG4。在两个患者组中观察到了相似的抗体结合模式。使用通过制备性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的变应原成分进行的抑制实验表明，在免疫印迹中检测到的梯牧草花粉的所有抗原成分都具有用于结合人IgE的特异性和交叉反应性决定簇。因此，用两种交叉反应成分进行减敏治疗后免疫治疗较差的临床结果与特异性抗体形成模式无关。
