Lorenzoni Fabio Cesar, Bonfante Estevam A, Bonfante Gerson, Martins Leandro M, Witek Lukasz, Silva Nelson R F A
Department of Prosthodontics, Bauru School of Dentistry, Brazil.
J Prosthodont. 2013 Aug;22(6):478-83. doi: 10.1111/jopr.12045. Epub 2013 Apr 1.
This evaluation aimed to (1) validate micro-computed tomography (microCT) findings using scanning electron microscopy (SEM) imaging, and (2) quantify the volume of voids and the bonded surface area resulting from fiber-reinforced composite (FRC) dowel cementation technique using microCT scanning technology/3D reconstructing software.
A fiberglass dowel was cemented in a condemned maxillary lateral incisor prior to its extraction. A microCT scan was performed of the extracted tooth creating a large volume of data in DICOM format. This set of images was imported to image-processing software to inspect the internal architecture of structures.
The outer surface and the spatial relationship of dentin, FRC dowel, cement layer, and voids were reconstructed. Three-dimensional spatial architecture of structures and volumetric analysis revealed that 9.89% of the resin cement was composed of voids and that the bonded area between root dentin and cement was 60.63% larger than that between cement and FRC dowel.
SEM imaging demonstrated the presence of voids similarly observed using microCT technology (aim 1). MicroCT technology was able to nondestructively measure the volume of voids within the cement layer and the bonded surface area at the root/cement/FRC interfaces (aim 2).
The interfaces at the root dentin/cement/dowel represent a timely and relevant topic where several efforts have been conducted in the past few years to understand their inherent features. MicroCT technology combined with 3D reconstruction allows for not only inspecting the internal arrangement rendered by fiberglass adhesively bonded to root dentin, but also estimating the volume of voids and contacted bond area between the dentin and cement layer.
本评估旨在（1）使用扫描电子显微镜（SEM）成像验证微型计算机断层扫描（microCT）的结果，以及（2）使用microCT扫描技术/3D重建软件量化纤维增强复合材料（FRC）桩核粘结技术产生的空隙体积和粘结表面积。
在拔除一颗已废弃的上颌侧切牙之前，将一根玻璃纤维桩核粘结于其中。对拔除的牙齿进行microCT扫描，生成大量DICOM格式的数据。将这组图像导入图像处理软件以检查结构的内部结构。
重建了牙本质、FRC桩核、粘结剂层和空隙的外表面及空间关系。结构的三维空间结构和体积分析显示，树脂粘结剂中有9.89%由空隙组成，牙根牙本质与粘结剂之间的粘结面积比粘结剂与FRC桩核之间的粘结面积大60.63%。
SEM成像显示存在空隙，这与使用microCT技术观察到的情况相似（目标1）。MicroCT技术能够无损测量粘结剂层内的空隙体积以及牙根/粘结剂/FRC界面处的粘结表面积（目标2）。
牙根牙本质/粘结剂/桩核界面是一个及时且相关的主题，在过去几年中人们已开展多项工作来了解其固有特征。MicroCT技术与3D重建相结合，不仅能够检查玻璃纤维与牙根牙本质粘结形成的内部结构，还能估计空隙体积以及牙本质与粘结剂层之间的接触粘结面积。
