KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
Antonie Van Leeuwenhoek. 2013 Jun;103(6):1385-93. doi: 10.1007/s10482-013-9919-z. Epub 2013 Apr 4.
A Gram-positive, coccoid to rod-shaped, non-spore-forming bacterium, designated Gsoil 958(T), was isolated from soil of a ginseng field located in Pocheon province in South Korea. This bacterium was characterized in order to determine its taxonomic position by using a polyphasic approach. Strain Gsoil 958(T) was observed to grow well at 25-30 °C and at pH 7.0 on R2A and nutrient agar without NaCl supplementation. Strain Gsoil 958(T) was determined to have β-glucosidase activity and the ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to F2 via gypenoside XVII and Rd. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 958(T) was shown to belong to the family Nocardioidaceae and related most closely to Nocardioides koreensis MSL-09(T) (97.6 % 16S rRNA gene sequence similarity), Nocardioides aquiterrae GW-9(T) (97.0 %), and Nocardioides sediminis MSL-01(T) (97.0 %). The sequence similarities with other validly named species within the genus Nocardioides were less than 96.8 %. Strain Gsoil 958(T) was characterized chemotaxonomically as having LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone, and iso-C16:0, iso-C16:1 H, iso-C14:0, iso-C15:0 were identified as the major fatty acids. The G + C content of genomic DNA was determined to be 70.8 mol %. The chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain Gsoil 958(T) to the genus Nocardioides. The results of both physiological and biochemical tests allowed for differentiation of strain Gsoil 958(T) from the recognized Nocardioides species. Therefore, strain Gsoil 958(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides panaciterrulae sp. nov. is proposed, with the type strain Gsoil 958(T) (KACC 14271(T) = KCTC 19471(T) = DSM 21350(T)).
一株革兰氏阳性、球形到杆状、非孢子形成的细菌，被命名为 Gsoil 958(T)，从位于韩国抱川省的人参田的土壤中分离出来。为了确定其分类地位，使用多相方法对该细菌进行了特征描述。菌株 Gsoil 958(T)在 25-30°C 和 pH 值为 7.0 的 R2A 和营养琼脂上生长良好，无需添加 NaCl。菌株 Gsoil 958(T)被确定具有β-葡萄糖苷酶活性，并能够通过ginsenoside XVII 和 Rd 将ginsenoside Rb1（人参的主要活性成分之一）转化为 F2。根据 16S rRNA 基因序列相似性，菌株 Gsoil 958(T)属于诺卡氏菌科，与 Nocardioides koreensis MSL-09(T)（97.6% 16S rRNA 基因序列相似性）、Nocardioides aquiterrae GW-9(T)（97.0%）和 Nocardioides sediminis MSL-01(T)（97.0%）最为密切相关。与属内其他已命名种的序列相似性均小于 96.8%。菌株 Gsoil 958(T)的化学分类特征为细胞壁肽聚糖中含有 LL-2,6-二氨基庚二酸，主要的menaquinone 为 MK-8(H4)，主要脂肪酸为 iso-C16:0、iso-C16:1 H、iso-C14:0、iso-C15:0。基因组 DNA 的 G+C 含量为 70.8 mol%。化学分类特性和表型特征支持菌株 Gsoil 958(T)属于诺卡氏菌属。生理生化试验的结果也使菌株 Gsoil 958(T)与已鉴定的诺卡氏菌种区分开来。因此，菌株 Gsoil 958(T)被认为代表了诺卡氏菌属的一个新种，命名为 Nocardioides panaciterrulae sp. nov.，其模式菌株为 Gsoil 958(T)（KACC 14271(T) = KCTC 19471(T) = DSM 21350(T)）。
