Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea.
Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea.
Antonie Van Leeuwenhoek. 2021 Jul;114(7):983-995. doi: 10.1007/s10482-021-01570-2. Epub 2021 Apr 17.
A novel Gram-staining-positive, short rod-shaped, non-motile, and non-pigmented actinobacterial strain (KIGAM211) was isolated from kaolinite, a soft white clay mineral, collected from Sancheong in the Republic of Korea. On the basis of 16S rRNA gene sequence analysis, strain KIGAM211 was determined to belong to the genus Nocardioides and was most closely related to N. ungokensis UKS-03 (97.5% similarity). Cells could grow between 4 and 35 °C (optimum 30 °C), 0-3% (w/v) NaCl concentration (optimum 0%) and pH 5.5-8.5 (optimum 7.0) on R2A agar. Morphological appearance of colonies was cream-white, arranged singly or in groups. Biochemical characterization of strain KAGAM211 indicated that it could hydrolyze casein, gelatin, Tweens 40 and tyrosine. Furthermore, the strain was positive for both oxidase and catalase activity. Strain KIGAM211 was characterized chemotaxonomically by MK-8 (H) as the predominant menaquinone and phosphatidylglycerol (PG) and phosphatidylinositol (PI) as the major polar lipids. Major fatty acids were iso-C and C ω9c. The Ortholog average nucleotide identity (OrthoANI) and in silico DNA-DNA hybridization (isDDH) values between strain KIGAM211T and its most closely related strains of the Nocardioides genus were < 82% and < 24%, respectively, suggesting that strain KIGAM211 represent a novel species. The whole genome size of KIGAM211 was 4.52 Mb, comprising a total of 4,294 genes with DNA G + C content of 72.3 mol%. The genome of strain KIGAM211 also comprises the biosynthetic gene cluster for alkylresorcinol as secondary metabolite. The results of physiological, taxonomical, phylogenetic, and whole genome analyses allowed for differentiation of strain KIGAM211 from the recognized Nocardioides species. Therefore, strain KIGAM211 is considered to represent a novel species, for which the name Nocardioides luti sp. nov. (type strain KIGAM211 = KCTC 49364 = JCM 33859) is proposed.
一株新型革兰氏阳性、短杆状、非运动、非色素产生放线菌菌株（KIGAM211）从韩国 Sancheong 采集的软白粘土矿物高岭石中分离得到。基于 16S rRNA 基因序列分析，菌株 KIGAM211 被确定属于诺卡氏菌属，与 N. ungokensis UKS-03（相似度 97.5%）最为密切相关。菌株 KIGAM211 可在 R2A 琼脂上于 4 至 35°C（最适 30°C）、0-3%（w/v）NaCl 浓度（最适 0%）和 pH5.5-8.5（最适 7.0）的条件下生长。菌落的形态外观为奶油白色，呈单生或丛生排列。菌株 KAGAM211 的生化特征表明其可水解酪蛋白、明胶、吐温 40 和酪氨酸。此外，该菌株氧化酶和过氧化氢酶活性均为阳性。菌株 KIGAM211 的化学分类特征为 MK-8（H）为主要的menaquinone，磷脂酰甘油（PG）和磷脂酰肌醇（PI）为主要的极性脂。主要脂肪酸为 iso-C 和 C ω9c。菌株 KIGAM211 与诺卡氏菌属中与其最密切相关的菌株的 Ortholog average nucleotide identity（OrthoANI）和 in silico DNA-DNA hybridization（isDDH）值分别<82%和<24%，表明菌株 KIGAM211 代表一个新种。KIGAM211 的全基因组大小为 4.52 Mb，共包含 4294 个基因，DNA G+C 含量为 72.3 mol%。菌株 KIGAM211 的基因组还包含烷基间苯二酚作为次生代谢物的生物合成基因簇。生理、分类、系统发育和全基因组分析的结果表明，菌株 KIGAM211 与已识别的诺卡氏菌属物种不同。因此，菌株 KIGAM211 被认为代表一个新种，建议将其命名为 Nocardioides luti sp. nov.（模式菌株 KIGAM211=KCTC 49364=JCM 33859）。
