Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3045-50. doi: 10.1099/ijs.0.010025-0. Epub 2009 Jul 30.
A Gram-positive, rod-shaped, non-spore-forming bacterium (Gsoil 1124(T)) was isolated from soil of a ginseng field of Pocheon province in South Korea, and was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 1124(T) was shown to belong to the family Nocardioidaceae and related to Nocardioides simplex (98.2 % 16S rRNA gene sequence similarity), Nocardioides aromaticivorans (98.1 %), Nocardioides nitrophenolicus (97.7 %) and Nocardioides kongjuensis (97.5 %). The sequence similarity with any other species with validly published names within the genus Nocardioides was less than 94.5 %. Strain Gsoil 1124(T) was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H(4)) as the predominant menaquinone and iso-C(16 : 0), C(18 : 0), C(16 : 0), and C(18 : 1)omega9c as the major fatty acids. The G+C content of the genomic DNA was 70.2 mol%. The chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain Gsoil 1124(T) to the genus Nocardioides. The results of physiological and biochemical tests, and the low level of DNA-DNA relatedness allowed genotypic differentiation of strain Gsoil 1124(T) from recognized Nocardioides species. Therefore, strain Gsoil 1124(T) is considered to represent a novel species, for which the name Nocardioides ginsengisoli sp. nov. is proposed, with the type strain Gsoil 1124(T) (=KCTC 19135(T)=CCUG 52478(T)=DSM 17921(T)).
一株革兰氏阳性、杆状、不产芽孢的细菌（Gsoil 1124(T)）从韩国抱川人参种植地的土壤中分离得到，并对其进行了特征描述，以确定其分类地位。基于 16S rRNA 基因序列相似性，菌株 Gsoil 1124(T)属于诺卡氏菌科，与 Nocardioides simplex（98.2% 16S rRNA 基因序列相似性）、Nocardioides aromaticivorans（98.1%）、Nocardioides nitrophenolicus（97.7%）和 Nocardioides kongjuensis（97.5%）最为相近。与诺卡氏菌属中其他具有有效发表名称的种的序列相似性均低于 94.5%。菌株 Gsoil 1124(T)的细胞壁肽聚糖中含有 ll-2,6-二氨基庚二酸，主要的醌类为 MK-8(H(4))，其主要脂肪酸为 iso-C(16:0)、C(18:0)、C(16:0)和 C(18:1)omega9c。基因组 DNA 的 G+C 含量为 70.2mol%。化学分类特征和表型特征支持菌株 Gsoil 1124(T)属于诺卡氏菌属。生理生化试验和低水平的 DNA-DNA 同源性结果表明，菌株 Gsoil 1124(T)与公认的诺卡氏菌种存在遗传分化。因此，菌株 Gsoil 1124(T)被认为代表了一个新的种，建议采用新的种名 Nocardioides ginsengisoli sp. nov.，其模式菌株为 Gsoil 1124(T)（=KCTC 19135(T)=CCUG 52478(T)=DSM 17921(T)）。
