Department of Medicine Division of Gastroenterology and Hepatology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA; Department of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Stem Cells. 2013 Sep;31(9):2024-30. doi: 10.1002/stem.1391.
Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings toward the development of novel diagnostics and therapies with direct clinical relevance. This study demonstrates that the cluster of differentiation genes CD24 and CD44 are differentially expressed across LGR5 positive "active" stem cells as well as HOPX positive "facultative" stem cells. Fluorescence-activated cell sorting enables differential enrichment of LGR5 (CD24-/CD44+) and HOPX (CD24+/CD44+) cells for gene expression analysis and culture. These findings provide the fundamental methodology and basic cell surface signature necessary for isolating and studying intestinal stem cell populations in human physiology and disease.
最近的开创性研究在小鼠模型中迅速推进了对肠上皮干细胞（IESC）生物学的理解。然而，缺乏适合从人体组织中分离和随后进行 IESC 下游分析的技术，这阻碍了这些发现应用于具有直接临床相关性的新型诊断和治疗方法的发展。本研究表明，簇分化基因 CD24 和 CD44 在 LGR5 阳性“活跃”干细胞以及 HOPX 阳性“兼性”干细胞中存在差异表达。荧光激活细胞分选可实现 LGR5（CD24-/CD44+）和 HOPX（CD24+/CD44+）细胞的差异富集，用于基因表达分析和培养。这些发现为分离和研究人类生理学和疾病中的肠干细胞群体提供了必要的基本方法和基本细胞表面特征。
