Department of Allergy, IIS-Fundación Jiménez Díaz, Madrid, Spain.
Mol Nutr Food Res. 2013 Jul;57(7):1283-90. doi: 10.1002/mnfr.201200332. Epub 2013 Apr 2.
IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain.
Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein.
We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.
过敏原的 IgE-表位作图揭示了参与过敏反应的抗原成分的重要信息。基于肽的微阵列免疫分析已被用于绘制一些食物过敏原的表位。我们开发了一种肽微阵列免疫分析方法，以绘制来自大西洋鳕鱼（Gad m 1）的副肌球蛋白中的变应原表位，大西洋鳕鱼是西班牙消费最多的鳕鱼品种。
使用了 13 名对鳕鱼副肌球蛋白特异性 IgE 的鱼类过敏患者的血清。合成了一个重叠肽文库，代表 Gad m 1 的一级序列。肽用于分析患者血清中的过敏原特异性 IgE 抗体。100%的患者识别出 Gad m 1 中 15 个氨基酸长的一个抗原区域。该区域与来自波罗的海鳕鱼（Gadus callarias）的副肌球蛋白 Gad c 1（过敏原 M）的三个抗原决定簇之一仅部分相关。在该蛋白质的 3D 模型中，该区域位于蛋白质表面。
我们已经确定了 Gad m 1 中的一个相关抗原区域。该表位可以被认为是严重程度的标志物，并提供了额外的信息，以改善鱼类过敏的诊断和安全免疫治疗工具的设计。
