Department of Immunotechnology, Lund University, Lund, Sweden.
Department of Otorhinolaryngology, Head & Neck Surgery, Skåne University Hospital, Lund, Sweden.
Front Immunol. 2024 Mar 5;15:1293793. doi: 10.3389/fimmu.2024.1293793. eCollection 2024.
Fish β-parvalbumins are common targets of allergy-causing immunity. The nature of antibody responses to such allergens determines the biological outcome following exposure to fish. Specific epitopes on these allergens recognised by antibodies are incompletely characterised.
High-content peptide microarrays offer a solution to the identification of linear epitopes recognised by antibodies. We characterized IgG and IgG4 recognition of linear epitopes of fish β-parvalbumins defined in the WHO/IUIS allergen database as such responses hold the potential to counter an allergic reaction to these allergens. Peripheral blood samples, collected over three years, of 15 atopic but not fish-allergic subjects were investigated using a microarray platform that carried every possible 16-mer peptide of known isoforms and isoallergens of these and other allergens.
Interindividual differences in epitope recognition patterns were observed. In contrast, reactivity patterns in a given individual were by comparison more stable during the 3 years-course of the study. Nevertheless, evidence of the induction of novel specificities over time was identified across multiple regions of the allergens. Particularly reactive epitopes were identified in the D helix of Cyp c 1 and in the C-terminus of Gad c 1 and Gad m 1.02. Residues important for the recognition of certain linear epitopes were identified. Patterns of differential recognition of isoallergens were observed in some subjects.
Altogether, comprehensive analysis of antibody recognition of linear epitopes of multiple allergens enables characterisation of the nature of the antibody responses targeting this important set of food allergens.
鱼类β-肌钙蛋白是常见的过敏原免疫靶标。针对此类过敏原的抗体反应性质决定了暴露于鱼类后的生物学后果。这些过敏原上被抗体识别的特定表位尚未完全确定。
高内涵肽微阵列为鉴定抗体识别的线性表位提供了一种解决方案。我们对 WHO/IUIS 过敏原数据库中定义的鱼类β-肌钙蛋白的线性表位的 IgG 和 IgG4 识别进行了表征，因为这些反应有可能对抗对这些过敏原的过敏反应。我们使用微阵列平台研究了 15 名过敏但非鱼类过敏的个体在三年内采集的外周血样本，该平台携带了这些和其他过敏原的已知同工型和同种异体的所有可能的 16 -mer 肽。
观察到个体间表位识别模式的差异。相比之下，在研究的 3 年过程中，个体的反应模式更为稳定。然而，在多个过敏原区域都发现了随时间诱导新特异性的证据。在 Cyp c 1 的 D 螺旋和 Gad c 1 和 Gad m 1.02 的 C 末端鉴定出特别反应性的表位。确定了某些线性表位识别的重要残基。在某些个体中观察到同种异体识别模式的差异。
总之，对多种过敏原线性表位的抗体识别进行全面分析，能够描述针对这组重要食物过敏原的抗体反应性质。
