University of Warmia and Mazury in Olsztyn, Chair of Food Biochemistry, Olsztyn-Kortowo, Poland.
Peptides. 2012 Nov;38(1):105-9. doi: 10.1016/j.peptides.2012.08.011. Epub 2012 Aug 23.
The aim of this study was to analyze the distribution of hexapeptide fragments considered as epitopes of Baltic cod parvalbumin beta (allergen Gad c 1) in the universal proteome. Cod (Gadus morhua subsp. callarias) parvalbumin hexapeptides cataloged in the Immune Epitope Database were used as query sequences. The UniProt database was screened using the WU-BLAST 2 program. The distribution of hexapeptide fragments was investigated in various protein families, classified according to the presence of the appropriate domains, and in proteins of plant, animal and microbial species. Hexapeptides from cod parvalbumin were found in the proteins of plants and animals which are food sources, microorganisms with various applications in food technology and biotechnology, microorganisms which are human symbionts and commensals as well as human pathogens. In the last case possible coverage between epitopes from pathogens and allergens should be avoided during vaccine design.
本研究旨在分析被认为是波罗的海鳕鱼 parvalbumin beta（过敏原 Gad c 1）表位的六肽片段在通用蛋白质组中的分布。使用免疫表位数据库中编目的鳕鱼（Gadus morhua subsp. callarias） parvalbumin 六肽作为查询序列。使用 WU-BLAST 2 程序筛选 UniProt 数据库。根据适当结构域的存在，对各种蛋白质家族进行分类，研究六肽片段的分布，并研究植物、动物和微生物物种的蛋白质。在食物来源的动植物蛋白质、在食品技术和生物技术中有各种应用的微生物、人类共生菌和共生菌以及人类病原体中发现了来自鳕鱼 parvalbumin 的六肽。在后一种情况下，在疫苗设计过程中应避免病原体和过敏原的表位之间可能的覆盖。
