Process Biochemistry, Biogen Idec, Cambridge, MA, 02142, USA.
Biotechnol Prog. 2013 May-Jun;29(3):825-8. doi: 10.1002/btpr.1717. Epub 2013 Apr 1.
Post-translational modifications can dramatically impact protein activity, but identifying such structure:function relationships, as well as capitalizing on functionally enhanced variants, is a significant challenge. Here, affinity chromatography resins that contained immobilized FcγRIII receptors were used to enrich nonfucosylated antibodies 6- to 9-fold, offering what may be a tractable method for both the identification of post-translational modifications that affect function, as well as a means to enrich variants with enhanced activity.
翻译后修饰可显著影响蛋白质活性，但识别此类结构与功能的关系以及利用功能增强的变体是一项重大挑战。在这里，含有固定化FcγRIII受体的亲和层析树脂被用于将非岩藻糖基化抗体富集6至9倍，这为识别影响功能的翻译后修饰提供了一种可行的方法，同时也是富集具有增强活性变体的一种手段。
