Discovery Sciences, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Pepparedsleden 1, Mölndal, 431 83, Sweden.
Sci Rep. 2016 Jun 30;6:28887. doi: 10.1038/srep28887.
Protein purification often involves affinity capture of proteins on stationary resin, alternatively proteins are captured on free flowing resin for subsequent separation from bulk fluid. Both methods require labour and time intensive separation of particulate matter from fluid. We present a method where affinity resin is contained within porous-walled containers, supporting clarification, product recovery, and concentration in a single step with minimal hands-on processing time, without significant investments in equipment.
蛋白质纯化通常涉及将蛋白质在固定树脂上进行亲和捕获，或者将蛋白质在自由流动的树脂上捕获，然后从大量流体中进行后续分离。这两种方法都需要耗费大量的劳动力和时间将颗粒物质从流体中分离出来。我们提出了一种方法，其中亲和树脂被包含在多孔壁容器中，支持澄清、产物回收和浓缩在一个步骤中，具有最小的手动处理时间，而无需在设备上进行重大投资。
