Yang Li, Zhu Huaijun, Liu Dongxiao, Liang Song, Xu Hao, Chen Jie, Wang Xuerong, Xu Zekuan
Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China;
J Biomed Res. 2011 Jul;25(4):246-53. doi: 10.1016/S1674-8301(11)60033-X.
Regular use of aspirin (ASA) could reduce the risk of gastric cancer although the precise mechanism remains unclear. Down-regulation of survivin may be one of the cyclooxygenase-independent mechanisms whereby ASA induces apoptosis of gastric cancer cell. In this study, we investigated the effect of ASA on the growth, apoptosis and survivin expression of gastric cancer cell line SGC7901. The survival of cells treated with 3.0 and 10.0 mmol/L ASA for 24 h was decreased by 44.6% and 88.5%, respectively. ASA at 3.0 mmol/L inhibited the viability of SGC7901 cells in a time-dependent manner. Apoptosis analysis showed similar results with MTT assay. ASA at 3.0 and 10.0 mmol/L decreased the mRNA transcript levels of survivin and reduced survivin protein levels in SGC7901 cells also in a time-dependent manner. Our findings indicated that ASA inhibited the proliferation of SGC7901 by suppressing survivin at both the transcriptional and translational level.
经常使用阿司匹林（ASA）可能会降低胃癌风险，尽管确切机制尚不清楚。生存素的下调可能是ASA诱导胃癌细胞凋亡的非环氧化酶依赖机制之一。在本研究中，我们研究了ASA对胃癌细胞系SGC7901生长、凋亡及生存素表达的影响。用3.0和10.0 mmol/L ASA处理细胞24小时后，细胞存活率分别降低了44.6%和88.5%。3.0 mmol/L的ASA以时间依赖性方式抑制SGC7901细胞的活力。凋亡分析与MTT试验结果相似。3.0和10.0 mmol/L的ASA也以时间依赖性方式降低SGC7901细胞中生存素的mRNA转录水平并降低生存素蛋白水平。我们的研究结果表明，ASA通过在转录和翻译水平抑制生存素来抑制SGC7901的增殖。
