Dong Hanzhang, Liu Gaogao, Jiang Biao, Guo Jiubing, Tao Guoquan, Yiu Wei, Zhou Jingsong, Li Guoxin
Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Department of General Surgery, The Affiliated Beijiao Hospital of Southern Medical University, Shunde, Guangdong 528311, P.R. China.
Biomed Rep. 2014 May;2(3):344-348. doi: 10.3892/br.2014.241. Epub 2014 Feb 26.
The purpose of this study was to determine the effect of aspirin plus cisplatin (CDDP) in the chemotherapy of gastric cancer. We cultured SGC7901/CDDP cells by long-term exposure of SGC7901 cells to small doses of CDDP . The cells were treated with aspirin, CDDP or aspirin plus CDDP for 24 h and cell growth was assessed by the MTT assay, the apoptotic rate by flow cytometry, the survivin mRNA expression by RT-PCR and the survivin protein expression by western blotting. The results revealed that the cell growth in the aspirin plus CDDP group was significantly inhibited. The apoptotic rate in the aspirin plus CDDP was significantly higher compared to that in the other groups. The survivin mRNA and protein expression were also significantly reduced in the aspirin plus CDDP group. Our data suggest that the combination of aspirin and CDDP exhibited a higher degree of toxicity against SGC7901/CDDP cells compared to that of aspirin or CDDP alone. Thus, the combination of aspirin plus CDDP may reduce the expression of survivin and induce the apoptosis of SGC7901/CDDP cells.
本研究的目的是确定阿司匹林联合顺铂（CDDP）在胃癌化疗中的作用。我们通过将SGC7901细胞长期暴露于小剂量CDDP来培养SGC7901/CDDP细胞。将细胞用阿司匹林、CDDP或阿司匹林联合CDDP处理24小时，通过MTT法评估细胞生长，通过流式细胞术评估凋亡率，通过RT-PCR评估生存素mRNA表达，通过蛋白质印迹法评估生存素蛋白表达。结果显示，阿司匹林联合CDDP组的细胞生长受到显著抑制。与其他组相比，阿司匹林联合CDDP组的凋亡率显著更高。阿司匹林联合CDDP组的生存素mRNA和蛋白表达也显著降低。我们的数据表明，与单独使用阿司匹林或CDDP相比，阿司匹林与CDDP联合对SGC7901/CDDP细胞表现出更高程度的毒性。因此，阿司匹林联合CDDP可能会降低生存素的表达并诱导SGC7901/CDDP细胞凋亡。
