Qiao Zhe, Dang Chengxue, Zhou Bin, Li Shaomin, Zhang Wei, Jiang Jiantao, Zhang Jin, Kong Ranran, Ma Yuefeng
Department of Thoracic Surgery, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China;
J Biomed Res. 2012 Jul;26(4):268-73. doi: 10.7555/JBR.26.20110121. Epub 2012 Jun 25.
The aim of this present study was to explore the expression and clinical significance of O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) and enzymatic O-linked glycosylation (O-GlcNAcation) through the addition of O-linked-β-N-acetylglucosamine in esophageal squamous cell carcinoma. OGT expression and O-GlcNAcation in 40 samples from patients with esophageal squamous cell carcinoma was detected by immunohistochemical staining with anti-OGT antib ody and O-GlcNAc-specific antibody RL2, respectively. The relationship between pathological and clinical factors of patients was analyzed. We found that the expression of OGT was higher in esophageal squamous cell carcinoma samples compared to the normal tissues. RL2 antibody level was positively correlated with OGT expression, and the metastasis of lymph node, which means the level of O-GlcNAcation was high and related to the metastasis of lymph node in esophageal squamous cell carcinoma. In conclusion, OGT activation is the main reason for promoting the level of O-GlcNAcation in esophageal squamous cell carcinoma. O-GlcNAcylation may play an important role in esophageal squamous cell carcinoma.
本研究旨在通过在食管鳞状细胞癌中添加O-连接的β-N-乙酰葡糖胺来探索O-连接的N-乙酰葡糖胺（O-GlcNAc）转移酶（OGT）的表达及临床意义和酶促O-连接糖基化（O-GlcNAcation）。分别用抗OGT抗体和O-GlcNAc特异性抗体RL2进行免疫组织化学染色，检测40例食管鳞状细胞癌患者样本中的OGT表达和O-GlcNAcation。分析患者病理和临床因素之间的关系。我们发现，与正常组织相比，食管鳞状细胞癌样本中OGT的表达更高。RL2抗体水平与OGT表达呈正相关，且与淋巴结转移相关，这意味着食管鳞状细胞癌中O-GlcNAcation水平较高且与淋巴结转移有关。总之，OGT激活是促进食管鳞状细胞癌中O-GlcNAcation水平的主要原因。O-GlcNAc糖基化可能在食管鳞状细胞癌中起重要作用。
