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花粉菊成分促进 PC12 细胞轴突生长，其作用机制与 p38MAPK 磷酸化抑制有关

Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. "Mottenohoka" Is Enhanced by Suppressing Phosphorylation of p38MAPK.

机构信息

Yonezawa Women's Junior College, 6-15-1 Tohrimachi, Yonezawa, Yamagata 992-0025, Japan.

出版信息

Evid Based Complement Alternat Med. 2013;2013:403503. doi: 10.1155/2013/403503. Epub 2013 Mar 10.

DOI:10.1155/2013/403503
PMID:23554829
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3608199/
Abstract

Components from Dendranthema × grandiflorum cv. "Mottenohoka" that promote neurite outgrowth of PC12 cells were identified and the mechanism of neurite outgrowth stimulated by isolated components was studied. Components that promoted the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK 1/2) of PC12 cells were isolated. From various structural analyses, the active components were identified as acacetin and luteolin. The effects of acacetin or luteolin on PC12 cells were evaluated by electro-blotting and immunostaining. Slight neurite outgrowth in PC12 cells was observed within 2 days of culture after stimulation by luteolin or acacetin. However, NGF-stimulation induced remarkable neurite outgrowth in comparison. Neurite outgrowth by luteolin or acacetin was significantly enhanced by pretreatment with SB203580 (a p38MAPK inhibitor). The results of this study into the phosphorylation of ERK 1/2 and p38MAPK by flavonoids suggest that the inhibition of p38MAPK phosphorylation may effectively enhance neurite outgrowth.

摘要

从菊花“毛团八重”中鉴定出促进 PC12 细胞突起生长的成分，并研究了分离成分刺激突起生长的机制。分离出促进 PC12 细胞细胞外信号调节激酶 1/2（ERK 1/2）磷酸化的成分。通过各种结构分析，鉴定出活性成分为木犀草素和木樨草素。通过电印迹和免疫染色评估木樨草素或木樨草素对 PC12 细胞的作用。在用木樨草素或木樨草素刺激培养 2 天后，PC12 细胞中可见轻微的突起生长。然而，与 NGF 刺激相比，诱导了明显的突起生长。用 SB203580（p38MAPK 抑制剂）预处理可显著增强木樨草素或木樨草素诱导的突起生长。本研究中对黄酮类化合物 ERK 1/2 和 p38MAPK 的磷酸化研究表明，抑制 p38MAPK 磷酸化可能有效增强突起生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/0d3656b3e7ee/ECAM2013-403503.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/3ed0bdc17e8d/ECAM2013-403503.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/b309d54da6fb/ECAM2013-403503.002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/fbee828077d1/ECAM2013-403503.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/a7ea0c912cfb/ECAM2013-403503.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/2f7c0773bd48/ECAM2013-403503.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/0d3656b3e7ee/ECAM2013-403503.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/3ed0bdc17e8d/ECAM2013-403503.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/b309d54da6fb/ECAM2013-403503.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/b61e9d721f3f/ECAM2013-403503.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/02ad028403c6/ECAM2013-403503.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/f5c03cb200b2/ECAM2013-403503.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/5be3938cdd71/ECAM2013-403503.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/fbee828077d1/ECAM2013-403503.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/a7ea0c912cfb/ECAM2013-403503.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/2f7c0773bd48/ECAM2013-403503.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5034/3608199/0d3656b3e7ee/ECAM2013-403503.010.jpg

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