菊花花朵中黄酮类化合物对人结肠癌Colon205细胞的细胞毒性活性。

Cytotoxic activity of flavonoids from the flowers of Chrysanthemum morifolium on human colon cancer Colon205 cells.

作者信息

Xie Yuan-Yuan, Yuan Dan, Yang Jing-Yu, Wang Li-Hui, Wu Chun-Fu

机构信息

Department of Traditional Chinese Medicines, Shenyang Pharmaceutical University, China.

出版信息

J Asian Nat Prod Res. 2009 Sep;11(9):771-8. doi: 10.1080/10286020903128470.

DOI:10.1080/10286020903128470

PMID:20183323

Abstract

A new p-hydroxyphenylacetyl flavonoid, diosmetin 7-(6''-O-p-hydroxyphenylacetyl)-O-beta-d-glucopyranoside (1), was isolated from the flowers of Chrysanthemum morifolium Ramat. 'huaiju' cv. nov. (Compositae), together with five known flavonoids, luteolin (2), diosmetin (3), diosmetin 7-O-beta-d-glucopyranoside (4), diosmin (5), and scolimoside (6), and four known caffeoylquinic acid derivatives, macranthoin F (7), 3,5-dicaffeoylquinic acid (8), 1,3-dicaffeoyl-epi-quinic acid (9), and chlorogenic acid (10). The structure of 1 was elucidated by UV, IR, ESI-TOF-MS, 1D, and 2D NMR spectroscopic methods. Cytotoxic activity of compounds 1-5 against human colon cancer cell Colon205 was investigated using MTT assays. Compounds 2 and 3 showed significant cytotoxicities against Colon205, with their IC(50) values being 96.9 and 82.9 microM, respectively. However, compounds 1, 4, and 5 showed little cytotoxic activity.

摘要

从菊花品种“怀菊”（菊科）的花中分离出一种新的对羟基苯乙酰黄酮，即香叶木素7 -（6''-O - 对羟基苯乙酰）-O-β -D -吡喃葡萄糖苷（1），同时还分离出五种已知的黄酮类化合物，木犀草素（2）、香叶木素（3）、香叶木素7 -O-β -D -吡喃葡萄糖苷（4）、地奥司明（5）和臭矢菜苷（6），以及四种已知的咖啡酰奎宁酸衍生物，大花金鸡菊素F（7）、3,5 -二咖啡酰奎宁酸（8）、1,3 -二咖啡酰-表奎宁酸（9）和绿原酸（10）。通过紫外光谱、红外光谱、电喷雾飞行时间质谱、一维和二维核磁共振光谱方法阐明了化合物1的结构。采用MTT法研究了化合物1 - 5对人结肠癌细胞Colon205的细胞毒性活性。化合物2和3对Colon205显示出显著的细胞毒性，其IC(50)值分别为96.9和82.9微摩尔。然而，化合物1、4和5显示出几乎没有细胞毒性活性。