Garelnabi Mahdi, Gupta Vinod, Mallika Venkatesan, Bhattacharjee Jayashree
Department of Clinical Laboratory and Nutritional Sciences, University of Massachusetts Lowell, Massachusetts, USA.
Ann Vasc Dis. 2011;4(2):99-105. doi: 10.3400/avd.oa.10.01024. Epub 2011 Jun 2.
Coronary artery disease (CAD) is the leading cause of death worldwide, and the major cause of hospital admissions in the Western countries. The pathogenesis of CAD is closely related to nitric oxide release and formation. The purpose of this study was to investigate the status of platelets nitric oxide in patients with coronary artery disease.
We measured platelets aggregation, cGMP, NO (nitrite/nitrate level), NO synthase activity, plasma NO, and ionized Ca(2+) in 40 healthy volunteers and 120 patients with myocardial infarction, unstable and stable angina, with 40 subjects in each group. The subjects' age mean range was from 40-51 years.
Platelets aggregation, NO, cGMP, NO synthase activity, plasma NO and ionized Ca(2+) have increased significantly (P <0.001) across the patients groups compared to controls. Platelets NO synthase activity (mean ± SD / U / 10(9) platelets) in healthy controls, MI, unstable angina and stable angina patients were 1.19 ± 0.56, 1.21 ± 0.64, 1.64 ± 0.98 and 1.57 ± 0.81 respectively. The cGMP (mean ± SD / pmole / 10(9) platelets) levels were 0.95 ± 0.41, 1.53 ± 0.64, 3.18 ± 0.77, and 5.12 ± 1.5 respectively.
The present study demonstrated that platelets aggregation, NO, cGMP, NO synthase activity, plasma NO, and ionized Ca(2+) profoundly increased in CAD. The increases in NO-cGMP components may have resulted as a compensatory response to ameliorate platelet activity and Ca(2+) levels in CAD patients.
冠状动脉疾病（CAD）是全球主要的死亡原因，也是西方国家住院的主要原因。CAD的发病机制与一氧化氮的释放和形成密切相关。本研究旨在调查冠心病患者血小板一氧化氮的状况。
我们测量了40名健康志愿者和120名心肌梗死、不稳定型和稳定型心绞痛患者的血小板聚集、环磷酸鸟苷（cGMP）、一氧化氮（亚硝酸盐/硝酸盐水平）、一氧化氮合酶活性、血浆一氧化氮和离子钙（Ca²⁺），每组40名受试者。受试者的年龄平均范围为40 - 51岁。
与对照组相比，各患者组的血小板聚集、一氧化氮、cGMP、一氧化氮合酶活性、血浆一氧化氮和离子钙（Ca²⁺）均显著增加（P <0.001）。健康对照组、心肌梗死患者、不稳定型心绞痛患者和稳定型心绞痛患者的血小板一氧化氮合酶活性（平均值±标准差/U/10⁹血小板）分别为1.19±0.56、1.21±0.64、1.64±0.98和1.57±0.81。cGMP（平均值±标准差/皮摩尔/10⁹血小板）水平分别为0.95±0.41、1.53±0.64、3.18±0.77和5.12±1.5。
本研究表明，CAD患者的血小板聚集、一氧化氮、cGMP、一氧化氮合酶活性、血浆一氧化氮和离子钙（Ca²⁺）显著增加。一氧化氮 - cGMP成分的增加可能是对CAD患者血小板活性和Ca²⁺水平改善的一种代偿反应。
