Ivarie R D, Fan W J, Tomkins G M
J Cell Physiol. 1975 Apr;85(2 Pt 2 Suppl 1):357-64. doi: 10.1002/jcp.1040850404.
Anucleate HTC cells have been used to determine the importance of the nucleus in the regulation of the intracellular levels of tyrosine aminotransferase (TAT) in hepatoma tissue culture (HTC) cells. In the absence of the nucleus, neither the induction of the enzyme by dexamethasone nor its deinduction upon removal of the hormone occurs. Degradation of the enzyme takes place when protein synthesis is inhibited in anucleates by cycloheximide. Therefore, the maintenance of induced levels of enzyme activity after dexamethasone withdrawal from pre-induced anucleates suggest that the nucleus is required for the inactivation of the TAT mRNA.
无核HTC细胞已被用于确定细胞核在肝癌组织培养（HTC）细胞中酪氨酸转氨酶（TAT）细胞内水平调节中的重要性。在没有细胞核的情况下，地塞米松对该酶的诱导作用以及去除激素后酶的去诱导作用均不会发生。当用环己酰亚胺抑制无核细胞中的蛋白质合成时，酶会发生降解。因此，从预先诱导的无核细胞中撤除地塞米松后，酶活性诱导水平的维持表明，细胞核是TAT mRNA失活所必需的。
