5α-雄甾烷-3α,17β-二醇在大鼠下颌下腺中的雄激素作用。

Androgenic actions of 5 alpha-androstane-3 alpha,17 beta-diol in rat submandibular gland.

作者信息

Furuyama M, Koshika S, Hayashi H, Ejiri H, Nakayama Y

机构信息

Department of Biochemistry, Kanagawa Dental College, Yokosuka, Japan.

出版信息

J Steroid Biochem. 1990 Apr;35(5):593-9. doi: 10.1016/0022-4731(90)90203-5.

DOI:10.1016/0022-4731(90)90203-5

PMID:2355733

Abstract

To evaluate the action of 5 alpha-androstane-3 alpha,17 beta-diol(3 alpha-diol) in rat submandibular gland, 5 alpha-reductase, 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSD) and oxidative 3 alpha-hydroxysteroid dehydrogenase (3 alpha-HSDO) activities, and trypsin-like protease activities, were assayed in control, castrated and 3 alpha-diol injected rats. 3 alpha-Diol (1 mg/kg) was injected subcutaneously in castrated male rats daily for 7 days. A 47% decrease of 5 alpha-reductase activity in the nuclei and a 30% decrease of 3 alpha-HSD(O) activities in the cytosol were shown after castration. 3 alpha-Diol restored the 5 alpha-reductase and 3 alpha-HSD(O) activities to 82 and 140% of the control submandibular gland, respectively. 3 alpha-Diol raised the trypsin-like protease activity to near control values in the submandibular gland of castrated rats. Morphological observations also revealed a distinct effect of 3 alpha-diol on the number of granules of granular duct cells. It is concluded that 3 alpha-diol has an androgenic action in the rat submandibular gland. It stimulates the 3 alpha-HSD(O). The 3 alpha-HSD(O) in its turn may be responsible for DHT accumulation in the cells.

摘要

为评估5α-雄甾烷-3α,17β-二醇（3α-二醇）在大鼠颌下腺中的作用，我们检测了对照、去势及注射3α-二醇的大鼠体内5α-还原酶、3α-羟基类固醇脱氢酶（3α-HSD）和氧化型3α-羟基类固醇脱氢酶（3α-HSDO）的活性以及类胰蛋白酶活性。对去势雄性大鼠每日皮下注射3α-二醇（1mg/kg），持续7天。去势后，细胞核中5α-还原酶活性降低47%，细胞质中3α-HSD（O）活性降低30%。3α-二醇可使5α-还原酶和3α-HSD（O）的活性分别恢复至对照颌下腺的82%和140%。3α-二醇还可使去势大鼠颌下腺中的类胰蛋白酶活性升高至接近对照值。形态学观察也显示3α-二醇对颗粒导管细胞的颗粒数量有显著影响。研究得出结论，3α-二醇在大鼠颌下腺中具有雄激素样作用，它可刺激3α-HSD（O），而3α-HSD（O）反过来可能是细胞内双氢睾酮（DHT）积累的原因。