Woldemeskel M W, Saliki J T, Blas-Machado U, Whittington L
University of Georgia, Tifton Veterinary Diagnostic and Investigational Laboratory, 43 Brighton Rd, Tifton, GA 31793, USA. Email:
Vet Pathol. 2013 Nov;50(6):989-93. doi: 10.1177/0300985813485097. Epub 2013 Apr 4.
The role of mast cells (MCs) in allergic reactions and parasitic infections is well established. Their involvement in host immune response against bacterial and viral infections is reported. In this study, investigation is made to determine if MCs are associated with Canine parvovirus-2 (CPV-2)-induced enteritis with crypt abscess (ECA). Mast cell count (MCC) was made on toluidine blue-stained intestinal sections from a total of 34 dogs. These included 16 dogs exhibiting ECA positive for CPV-2 and negative for Canine distemper virus and Canine coronavirus by immunohistochemistry and fluorescent antibody test, 12 dogs with inflammatory bowel disease (IBD), and 6 non-ECA/non-IBD (control) dogs. The average total MCC per high-power field in ECA (40.8 ± 2.2) and IBD (24.7 ± 2.1) was significantly higher (P < .05) than in the control (3.4 ± 0.6). Although not significant (P > .05), MCC was also higher in ECA than in IBD. The present study for the first time has documented significantly increased MCs in CPV-2-associated ECA as was previously reported for IBD, showing that MCs may also play an important role in CPV-2-associated ECA. Further studies involving more CPV-infected dogs are recommended to substantiate the findings.
肥大细胞（MCs）在过敏反应和寄生虫感染中的作用已得到充分证实。据报道，它们也参与宿主针对细菌和病毒感染的免疫反应。在本研究中，我们进行了调查，以确定MCs是否与犬细小病毒2型（CPV - 2）引起的伴有隐窝脓肿的肠炎（ECA）有关。对总共34只犬的甲苯胺蓝染色肠道切片进行了肥大细胞计数（MCC）。其中包括16只通过免疫组织化学和荧光抗体检测显示CPV - 2呈阳性、犬瘟热病毒和犬冠状病毒呈阴性的ECA阳性犬，12只患有炎症性肠病（IBD）的犬，以及6只非ECA/非IBD（对照）犬。ECA组（40.8±2.2）和IBD组（24.7±2.1）每高倍视野的平均总MCC显著高于对照组（3.4±0.6）（P <.05）。虽然差异不显著（P>.05），但ECA组的MCC也高于IBD组。本研究首次记录了CPV - 2相关ECA中MCs显著增加，正如之前在IBD中所报道的那样，表明MCs可能在CPV - 2相关ECA中也发挥重要作用。建议进行更多涉及CPV感染犬的进一步研究以证实这些发现。
