Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso, Showa, Nagoya, Japan.
J Sep Sci. 2013 Jun;36(12):1980-5. doi: 10.1002/jssc.201201191. Epub 2013 May 16.
A method of simultaneous separation of water- and fat-soluble vitamins using pressure-assisted CEC with a methacrylate-based capillary monolithic column was developed. In the proposed method, water-soluble vitamins were mainly separated electrophoretically, while fat soluble-ones were separated chromatographically by the interaction with a methacrylate-based monolith. A mixture of six water-soluble and four fat-soluble vitamins was separated simultaneously within 20 min with an isocratic elution using 1 M formic acid (pH 1.9)/acetonitrile (30:70, v/v) containing 10 mM ammonium formate as a mobile phase. When the method was applied to a commercial multivitamin tablet and a spiked one, the vitamins were successfully analyzed, and no influence of the matrix contained in the tablet was observed.
采用基于甲基丙烯酸酯的毛细管整体柱的加压毛细管电泳同时分离水溶性和脂溶性维生素的方法。在该方法中，水溶性维生素主要通过电泳分离，而脂溶性维生素则通过与基于甲基丙烯酸酯的整体柱相互作用进行色谱分离。使用 1 M 甲酸（pH 1.9）/乙腈（30:70，v/v）含 10 mM 甲酸铵作为流动相进行等度洗脱，可在 20 分钟内同时分离六种水溶性和四种脂溶性维生素。当该方法应用于商业复合维生素片剂和加标片剂时，成功地分析了维生素，并且未观察到片剂中所含基质的影响。
