Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2013;6:267-85. doi: 10.1146/annurev-anchem-062012-092700. Epub 2013 Apr 1.
Infrared (IR) spectroscopy of biomolecular ions combines mass spectrometry's high sensitivity and ability to analyze complex mixtures with the enhanced structural information available from vibrational spectroscopy. IR spectroscopy is in principle well placed to distinguish isomers and allow chemical classification of unknown molecules. This review gives an outline of current instrumentation, spectroscopic approaches, and potential bottlenecks. We discuss the most promising applications in bioanalytical mass spectrometry in view of recent experimental results, as well as future applications based on bioinformatics.
生物分子离子的红外（IR）光谱学将质谱的高灵敏度和分析复杂混合物的能力与振动光谱学提供的增强结构信息相结合。IR 光谱学原则上非常适合区分异构体并允许对未知分子进行化学分类。本综述概述了当前的仪器仪表、光谱方法和潜在的瓶颈。我们根据最近的实验结果讨论了在生物分析质谱学中最有前途的应用，以及基于生物信息学的未来应用。
