Bao Yuhua, Yanase Emiko, Nakatsuka Shin-ichi
The United Graduate School of Agricultural Science, Gifu University.
Biosci Biotechnol Biochem. 2013;77(4):877-9. doi: 10.1271/bbb.120938. Epub 2013 Apr 7.
Campesteryl ferulate (3a, 24R/α) and epi-campesteryl ferulate (3b, 24S/β), components of rice bran γ-oryzanol, were isolated by the preparative recycle HPLC system using a combination of ODS silica and cholester packed columns at over 99% purity. Their purities and structures of 3a and 3b thus obtained were confirmed by HPLC analysis and physical data (1H- and 13C-NMR, MS spectra, and X-ray crystallography).
通过使用ODS硅胶柱和胆固醇填充柱组合的制备循环高效液相色谱系统，分离得到米糠γ-谷维素的成分阿魏酸菜油甾醇酯（3a，24R/α）和表阿魏酸菜油甾醇酯（3b，24S/β），纯度超过99%。通过高效液相色谱分析和物理数据（1H-和13C-核磁共振、质谱和X射线晶体学）确认了由此获得的3a和3b的纯度和结构。
