Mawhinney M G, Belis J A, Thomas J A, Lloyd J W
J Pharmacol Exp Ther. 1975 Jan;192(1):242-9.
The in vitro actions of prolactin on the metabolism of testosterone by the guinea-pig liver and sex accessory organs have been investigated under a variety of conditions including various concentrations of ovine or bovine prolactin, addition of reduced nicotinamide adenine dinucleotide phosphatate (NADPH) or nicotinamide adenine dinucleotide (NAD) to incubation media and various lengths of incubations. Treatment of the epithelium and muscle of the guinea-pig seminal vesicle with NAD elicited a shift from normal reductive metabolism of testosterone toward oxidative metabolism. This shift toward oxidative metabolism of testosterone was particularly marked in the muscle of the guinea-pig seminal vesicle. Ovine prolactin was highly effective in inhibiting the reductive metabolism of testosterone by the sex accessory organs. This effect appeared primarily as a decrease in dihydrotestosterone formation and was noted in both the presence and absence of NADPH or NAD. Oxidized metabolites, androstenedione and androstandione, were apparently insensitive to prolactin treatment in vitro. Variations induced by prolactin in the concentrations of intracellular androgens may be important in either the normal or abnormal growth and function of male sex accessory organs.
在多种条件下，包括不同浓度的绵羊或牛催乳素、向孵育介质中添加还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）或烟酰胺腺嘌呤二核苷酸（NAD）以及不同的孵育时长，研究了催乳素对豚鼠肝脏和性附属器官睾酮代谢的体外作用。用NAD处理豚鼠精囊的上皮和肌肉，会使睾酮的正常还原代谢向氧化代谢转变。这种向睾酮氧化代谢的转变在豚鼠精囊的肌肉中尤为明显。绵羊催乳素在抑制性附属器官对睾酮的还原代谢方面非常有效。这种作用主要表现为二氢睾酮形成的减少，在有或没有NADPH或NAD的情况下均有此现象。氧化代谢产物雄烯二酮和雄烷二酮在体外显然对催乳素处理不敏感。催乳素引起的细胞内雄激素浓度变化可能对雄性性附属器官的正常或异常生长及功能很重要。
