Department of Otorhinolaryngology and Head and Neck Surgery, First Faculty of Medicine, Charles University in Prague, Motol University Hospital, Prague, Czech Republic.
Int J Cancer. 2013 Oct 15;133(8):1832-9. doi: 10.1002/ijc.28194. Epub 2013 May 2.
The purpose of this study was to determine whether changes in human papillomavirus (HPV) DNA prevalence in oral rinses and/or HPV-specific antibody levels in the sera of patients with oral/oropharyngeal cancer have prognostic significance. One hundred and forty-two patients with oral/oropharyngeal tumors were enrolled. The presence of HPV DNA was assayed in tumor tissue and oral rinses and HPV-specific antibodies were assessed in the sera. Oral rinses were collected before treatment and one year after the treatment. Sera were drawn before treatment, one month, and one year after the end of the treatment. Altogether, 59.2% of tumors were HPV positive. The presence of HPV DNA in the tumors correlated with HPV DNA positivity in oral rinses and with HPV-specific antibodies in the sera. Out of 66 patients with HPV-positive oral rinses at enrolment, 84.8% became negative at one-year follow-up, while most patients remained seropositive for HPV-specific antigens. However, the mean titers of HPV16 E6 and/or E7 antibodies at follow-up were significantly lower. Of 16 patients with recurrences at follow-up (alive on second sampling), six were positive at enrolment for HPV16 E6 and/or E7 antibodies. In five of these, no decrease in antibody levels was observed. Titers of antibodies specific for HPV16 capsid antigens did not change during the follow-up. Our data suggest that the detection of antibodies specific for the HPV 16 E6 and E7 oncoproteins may serve not only as a marker of HPV etiology, but also as a marker of recurrence and a prognostic indicator in patients with HPV-positive tumors.
本研究旨在确定口腔冲洗液中人乳头瘤病毒 (HPV) DNA 流行率的变化和/或口腔/口咽癌患者血清中 HPV 特异性抗体水平的变化是否具有预后意义。共纳入 142 例口腔/口咽肿瘤患者。检测肿瘤组织和口腔冲洗液中的 HPV DNA，并评估血清中的 HPV 特异性抗体。在治疗前和治疗后一年采集口腔冲洗液，在治疗前、治疗后一个月和一年采集血清。总共，59.2%的肿瘤呈 HPV 阳性。肿瘤中 HPV DNA 的存在与口腔冲洗液中的 HPV DNA 阳性和血清中的 HPV 特异性抗体相关。在 66 例入组时 HPV 阳性口腔冲洗液的患者中，84.8%在一年随访时转为阴性，而大多数患者仍对 HPV 特异性抗原呈血清阳性。然而，随访时 HPV16 E6 和/或 E7 抗体的平均滴度显著降低。在随访中发现 16 例复发患者（第二次采样时存活），6 例在入组时 HPV16 E6 和/或 E7 抗体阳性。在这 6 例中，有 5 例未观察到抗体水平下降。针对 HPV16 衣壳抗原的特异性抗体的滴度在随访期间没有变化。我们的数据表明，针对 HPV16 E6 和 E7 癌蛋白的特异性抗体的检测不仅可以作为 HPV 病因的标志物，还可以作为 HPV 阳性肿瘤患者复发和预后的标志物。
