School of Chemistry, National University of Ireland Galway, Galway, Ireland.
J Biomater Sci Polym Ed. 2013;24(3):253-68. doi: 10.1080/09205063.2012.690274. Epub 2012 Aug 14.
A series of poly-(N-isopropyl acrylamide)-based copolymers were developed with a view to biomedical applications, specifically cell cultivation and recovery. Ethylpyrrolidone methacrylate (EPM), the monomer of poly-(ethylpyrrolidone methacrylate) (pEPM), which is itself thermoresponsive, was copolymerized with N-isopropylacrylamide in varying ratios to create this novel thermoresponsive copolymer series. Characterization indicated a moderate increase of copolymer lower critical solution temperature with increasing EPM content. Films of the copolymers successfully hosted cells to monolayer. Cells detached from the copolymers upon temperature reduction with cell to cell junctions maintained, avoiding the damage which can be caused using conventional detachment techniques. These results indicate that these copolymers are highly cell compatible and may be useful for a range of biomedical applications.
一系列基于聚（N-异丙基丙烯酰胺）的共聚物被开发出来，旨在应用于生物医学领域，特别是细胞培养和回收。甲基丙烯酰基乙基吡咯烷酮（EPM）是聚（甲基丙烯酰基乙基吡咯烷酮）（pEPM）的单体，本身具有温敏性，与 N-异丙基丙烯酰胺共聚，形成了这种新型温敏性共聚物系列。表征表明，共聚物的下临界溶液温度随 EPM 含量的增加而适度升高。共聚物薄膜成功地培养了单层细胞。通过降低温度使细胞从共聚物上脱落，同时保持细胞间连接，避免了使用传统的分离技术可能造成的损伤。这些结果表明，这些共聚物具有高度的细胞相容性，可能对一系列生物医学应用有用。
