Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology (TUL), Lodz, Poland.
Int J Biol Macromol. 2013 Jul;58:148-53. doi: 10.1016/j.ijbiomac.2013.03.070. Epub 2013 Apr 6.
Catalases, heme enzymes, which catalyze decomposition of hydrogen peroxide to water and molecular oxygen, belong to the antioxidant defense system of the cell. In this work we have shown that catalase from bovine liver is inhibited by flavonoids. The inhibition is, at least partially, due to the formation of hydrogen bonds between catalase and flavonoids. In the presence of some flavonoids the formation of unreactive catalase compound II has been detected. The most potent catalase inhibitors among the tested flavonoids have appeared myricetin, epicatechin gallate and epigallocatechin gallate. The relationship between the degree of enzyme inhibition and molecular structure of flavonoids has been analyzed.
过氧化氢酶是血红素酶，能将过氧化氢分解为水和分子氧，属于细胞抗氧化防御系统。在这项工作中，我们已经证明牛肝过氧化氢酶可被类黄酮抑制。这种抑制至少部分是由于过氧化氢酶与类黄酮之间形成氢键所致。在某些类黄酮存在的情况下，已检测到无活性的过氧化氢酶复合物 II 的形成。在所测试的类黄酮中，杨梅素、没食子儿茶素没食子酸酯和表没食子儿茶素没食子酸酯是最有效的过氧化氢酶抑制剂。分析了酶抑制程度与类黄酮分子结构之间的关系。
