Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Bioresour Technol. 2013 May;136:131-9. doi: 10.1016/j.biortech.2013.02.056. Epub 2013 Feb 26.
This study investigates the potential for extracting sugars from the polysaccharides of Eastern redcedar. Pretreatment temperature, time, sulfuric acid loading, sodium bisulfite loading and impregnation time were varied using factorial treatment design experiments for identifying near optimal overall wood glucan-to-glucose yields during acid bisulfite pretreatments. The highest overall wood glucan-to-glucose yield of 87% was achieved when redcedar was impregnated with pretreatment liquor containing 3.75 g of sulfuric acid/100g of dry wood and 20 g of sodium bisulfite/100g of dry wood at 90 °C for 3h followed by increasing the temperature to 200 °C with a hold time of 10 min. Hemicellulose and lignin removal during pretreatments made the substrate amenable to enzymatic hydrolysis using 0.5 ml of Accelerase® 1500/g of glucan at 2% (w/w) solid loading. Preliminary mass balances showed 97% glucan recovery at pretreatment condition with 87% overall wood glucan-to-glucose yield and 59% delignification.
本研究探讨了从东方红杉的多聚糖中提取糖的可能性。通过使用析因处理设计实验，对预处理温度、时间、硫酸负载量、亚硫酸氢钠负载量和浸渍时间进行了变化，以确定在酸亚硫酸氢盐预处理过程中获得最大的整体木材葡聚糖至葡萄糖收率的最佳条件。当红杉用预处理液浸渍，其中含有 3.75 g 硫酸/100g 干木和 20 g 亚硫酸氢钠/100g 干木，在 90°C 下处理 3 小时，然后升温至 200°C 并保持 10 分钟时，整体木材葡聚糖至葡萄糖的收率最高可达 87%。预处理过程中半纤维素和木质素的去除使底物在 2%（w/w）固体负荷下使用 0.5ml 的 Accelerase® 1500/g 葡聚糖进行酶水解成为可能。初步的质量平衡显示，在预处理条件下，97%的葡聚糖得到回收，整体木材葡聚糖至葡萄糖的收率为 87%，木质素脱除率为 59%。
