Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Department of Biosystems and Agricultural Engineering, Oklahoma State University, Stillwater, OK 74078, USA.
Bioresour Technol. 2014 Jun;161:1-9. doi: 10.1016/j.biortech.2014.03.005. Epub 2014 Mar 12.
This study investigated the effect of two wood zones (sapwood versus heartwood) and size reduction techniques [Crumbles® (Crumbles® is a registered trademark of Forest Concepts, LLC, Auburn, WA, USA) particles versus ground particles] on wood glucan-to-ethanol yield after acid bisulfite pretreatment and simultaneous saccharification and fermentation (SSF) of Eastern redcedar. SSFs were conducted at 8% solids loading (w/w dry basis) using Accellerase® 1500 at a loading of 46FPU/g glucan and Saccharomyces cerevisiae D5A for ethanol fermentation. The size reduction technique had no effect on ethanol yield. However, sapwood glucan-to-ethanol yields were significantly greater than heartwood yields. The highest wood glucan-to-ethanol yield of 187L/dryMg (95% of theoretical) was achieved with sapwood crumbled particles in 240h. Ground sapwood, crumbled heartwood and ground heartwood achieved ethanol yields of 89%, 81% and 80% of theoretical in 240h, respectively. Preliminary mass balances showed 100% glucan recovery with crumbled sapwood and extensive (72%) delignification.
本研究考察了两种木材区域（边材与心材）和减小粒径技术[Crumbles®（Crumbles®是美国华盛顿州奥本的 Forest Concepts, LLC 的注册商标）颗粒与粉碎颗粒]对酸亚硫酸盐预处理后和同时糖化发酵（SSF）东方红柏木聚糖-乙醇产率的影响。在 8%固含率（w/w 干基）下，使用 Accellerase®1500 进行 SSF，其用量为 46FPU/g 葡聚糖和酿酒酵母 D5A 进行乙醇发酵。粒径减小技术对乙醇产率没有影响。然而，边材木聚糖-乙醇产率明显大于心材产率。在用边材粉碎颗粒进行的 240 小时实验中，最高的木材木聚糖-乙醇产率达到了 187L/dryMg（95%的理论值）。在 240 小时内，粉碎的心材、粉碎的边材和粉碎的边材分别实现了 89%、81%和 80%的理论乙醇产率。初步质量平衡显示，粉碎边材的木聚糖回收率为 100%，且木质素去除率达到 72%。
