Enzyme Technology Laboratory, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil.
Bioresour Technol. 2013 May;136:288-94. doi: 10.1016/j.biortech.2013.03.009. Epub 2013 Mar 14.
Ozonolysis was studied separately and in combination with wet disk milling (WDM) for the pretreatment of sugarcane bagasse and straw, with the aim of improving their enzymatic saccharification. The glucose yields for ozonolysis followed by WDM were 89.7% for bagasse and 63.1% for straw, whereas the use of WDM followed by ozonolysis resulted in glucose yields of 81.1% for bagasse and 92.4% for straw, with shorter WDM time. This last procedure allowed a substantial decrease in energy consumption in comparison to the use of WDM alone or of ozonolysis followed by WDM. Higher overall saccharification yields with shorter milling times were observed when ozonolysis was carried out before WDM. This effect might be related to the higher specific surface area. Additionally, a finer morphology was observed by the association of the two treatments in comparison to the sole use of ozonolysis or WDM.
臭氧氧化法分别与湿式球磨（WDM）联合预处理甘蔗渣和秸秆，旨在提高其酶解糖化效率。臭氧氧化法与湿式球磨联合预处理后，蔗渣的葡萄糖得率为 89.7%，秸秆为 63.1%；而湿式球磨法与臭氧氧化法联合预处理后，蔗渣的葡萄糖得率为 81.1%，秸秆为 92.4%，且湿式球磨时间更短。与单独使用湿式球磨或臭氧氧化法与湿式球磨联合使用相比，该方法可显著降低能耗。与单独使用湿式球磨或臭氧氧化法与湿式球磨联合使用相比，先进行臭氧氧化法处理后再进行湿式球磨，可获得更高的总糖化效率和更短的球磨时间。这种效果可能与更高的比表面积有关。此外，与单独使用臭氧氧化法或湿式球磨相比，联合处理后的形态更精细。
