State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2015 Mar;179:444-451. doi: 10.1016/j.biortech.2014.12.063. Epub 2014 Dec 29.
Two clean pretreatments, ozonolysis (OZ) and planetary ball milling (BM) were applied separately and in combination to improve the enzymatic hydrolysis of corn straw. Pretreatment of corn straw by OZ and BM alone improved the enzymatic hydrolysis significantly, primarily through delignification and decrystallization of cellulose, respectively. When combined, OZ-BM and BM-OZ pretreatments made the enzymatic hydrolysis more efficient. The glucose and xylose yield of corn straw treated with OZ for 90 min followed by BM for 8 min (OZ90-BM8) reached to 407.8 and 101.9 mg/g-straw, respectively under cellulase loading of 15 FPU/g-straw, which was fivefold more than that of untreated straw. Under much lower cellulase loading of 1.5 FPU/g-straw, the glucose and xylose yield of treated straw OZ90-BM8 remained at 416.0 and 108.4 mg/g-straw, respectively, while the yield of untreated straw decreased. These findings indicate that the combined OZ-BM can be used as a promising pretreatment for corn straw.
两种清洁预处理方法，臭氧化（OZ）和行星球磨（BM），分别单独和组合使用，以提高玉米秸秆的酶解效率。单独使用 OZ 和 BM 预处理玉米秸秆可以显著提高酶解效率，主要是通过分别脱木质素和纤维素结晶。当组合使用时，OZ-BM 和 BM-OZ 预处理使酶解更有效。在纤维素酶用量为 15 FPU/g-秸秆的条件下，经 OZ 处理 90 分钟后再用 BM 处理 8 分钟（OZ90-BM8）的玉米秸秆的葡萄糖和木糖产率分别达到 407.8 和 101.9 mg/g-秸秆，分别是未处理秸秆的五倍。在纤维素酶用量低得多的 1.5 FPU/g-秸秆条件下，处理过的 OZ90-BM8 秸秆的葡萄糖和木糖产率分别保持在 416.0 和 108.4 mg/g-秸秆，而未处理秸秆的产率则下降。这些发现表明，联合 OZ-BM 可作为玉米秸秆的一种有前途的预处理方法。
