Department of Anatomy, School of Life Dentistry at Tokyo, Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102 -8159, Japan.
Eur Arch Otorhinolaryngol. 2014 May;271(5):905-11. doi: 10.1007/s00405-013-2469-1. Epub 2013 Apr 9.
Substance P-immunoreactive nerve fiber (SP-IR NF) and calcitonin gene-related peptide-immunoreactive nerve fiber (CGRP-IR NF) are important mediators of neurogenic inflammation and blood supply. SP-IR and CGRP-IR NFs in the tensor tympani muscle (TTM) of the human middle ear have yet to be described. In this study, the TTM, tympanic membrane, malleus in the middle ear and tensor veli palatini muscle (TVPM) were examined by whole-mount immunohistochemistry in tissue from Japanese subjects. Thirteen human cadavers (ranging in age from 46 to 90 years) were used in this study. SP-IR and CGRP-IR NFs were primarily found on vessels at the origin, insertion and belly of the surface of the TTM and on the internal surface of the tympanic membrane. These neural factors were also detected on the surface of the malleus and the insertion of the TVPM. Therefore, our results indicate that existence of the SP-IR and CGRP-IR NFs of the TTM and the TVPM may reflect muscle properties involved in pain or inflammation of the middle ear.
P 物质免疫反应性神经纤维（SP-IR NF）和降钙素基因相关肽免疫反应性神经纤维（CGRP-IR NF）是神经源性炎症和血液供应的重要介质。人类中耳的鼓膜张肌（TTM）中的 SP-IR 和 CGRP-IR NF 尚未被描述。在这项研究中，通过对来自日本受试者的组织进行全组织免疫组织化学检查了中耳的 TTM、鼓膜、锤骨和腭帆张肌（TVPM）。这项研究使用了 13 个人体尸体（年龄在 46 至 90 岁之间）。SP-IR 和 CGRP-IR NF 主要存在于 TTM 表面的起点、插入点和腹部以及鼓膜内表面的血管上。这些神经因子也在锤骨和 TVPM 的插入部位被检测到。因此，我们的结果表明 TTM 和 TVPM 的 SP-IR 和 CGRP-IR NF 的存在可能反映了中耳疼痛或炎症相关的肌肉特性。
