Hauser-Kronberger C, Hacker G W, Franz P, Albegger K, Dietze O
Institute of Pathological Anatomy, Immunohistochemistry and Biochemistry Unit, Salzburg County General Hospital, Salzburg, Austria.
Regul Pept. 1997 Oct 31;72(2-3):79-85. doi: 10.1016/s0167-0115(97)01040-9.
The distribution of intraepithelial nerve fibres and neuroendocrine cells within the surface and glandular epithelium of human nasal mucosa and larynx was examined using immunohistochemical techniques. Neuronal structures were immunostained for the general neuroendocrine marker protein gene-product (PGP) 9.5, and the two neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) using immunofluorescence and streptavidin-biotin-peroxidase complex (S-ABC) methods. Intraepithelial nerve fibres with free nerve endings contained PGP 9.5 and were found within the respiratory surface epithelium of the nasal mucosa and the squamous epithelium of the larynx. A subpopulation of these nerve fibres showed positive immunoreactivties with antibodies against SP and CGRP. Nerve fibres within the ductal epithelium of subepithelial excretory ducts passing the basal membrane and reaching the luminal part were detected. These nerve fibres showed CGRP-like immunoreactivity but not for SP. A dense network of nerve fibres within the squamous surface epithelium was detected in the subglottic and epiglottic region containing CGRP and SP in a small subpopulation of nerve fibres. Single intraepithelial taste buds in the epiglottic region and neuroendocrine cells within the subglottic epithelium expressed PGP 9.5.
运用免疫组织化学技术，对人鼻黏膜和喉的表面及腺上皮内的上皮内神经纤维和神经内分泌细胞的分布进行了研究。采用免疫荧光和链霉亲和素-生物素-过氧化物酶复合物（S-ABC）方法，对神经元结构进行免疫染色，以检测一般神经内分泌标志物蛋白基因产物（PGP）9.5以及两种神经肽P物质（SP）和降钙素基因相关肽（CGRP）。含有游离神经末梢的上皮内神经纤维含有PGP 9.5，见于鼻黏膜的呼吸表面上皮和喉的鳞状上皮内。这些神经纤维的一个亚群对抗SP和CGRP的抗体呈阳性免疫反应。检测到穿过基底膜并到达管腔部分的上皮下排泄管的导管上皮内的神经纤维。这些神经纤维显示出CGRP样免疫反应，但对SP无反应。在声门下和会厌区域的鳞状表面上皮内检测到一个密集的神经纤维网络，一小部分神经纤维含有CGRP和SP。会厌区域的单个上皮内味蕾和声门下上皮内的神经内分泌细胞表达PGP 9.5。
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