Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China.
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China; Center for Quantitative Biology, Peking University, Beijing 100871, China.
J Biol Chem. 2013 May 17;288(20):14384-14390. doi: 10.1074/jbc.M112.441501. Epub 2013 Apr 8.
Cytokinesis is the final stage of cell division in which the cytoplasm of a cell is divided into two daughter cells after the segregation of genetic material, and the central spindle and midbody are considered to be the essential structures required for the initiation and completion of cytokinesis. Here, we determined that the centrosome protein Cep57, which is localized to the central spindle and midbody, acts as a spindle organizer and is required for cytokinesis. Depletion of Cep57 disrupted microtubule assembly of the central spindle and further led to abnormal midbody localization of MKLP1, Plk1, and Aurora B, which resulted in cytokinesis failure and the formation of binuclear cells. Furthermore, we found that Cep57 directly recruited Tektin 1 to the midbody matrix to regulate microtubule organization. Thus, our data reveal that Cep57 is essential for cytokinesis via regulation of central spindle assembly and formation of the midbody.
有丝分裂是细胞分裂的最后阶段，在遗传物质分离后，细胞质被分成两个子细胞，中心纺锤体和中体被认为是启动和完成有丝分裂所必需的结构。在这里，我们确定定位于中心纺锤体和中体的中心体蛋白 Cep57 作为纺锤体组织者发挥作用，并且是有丝分裂所必需的。Cep57 的耗竭破坏了中心纺锤体的微管组装，并进一步导致 MKLP1、Plk1 和 Aurora B 的异常中体定位，导致有丝分裂失败和双核细胞的形成。此外，我们发现 Cep57 直接将 Tektin 1 募集到中体基质中以调节微管组织。因此，我们的数据表明，Cep57 通过调节中心纺锤体的组装和中体的形成，对有丝分裂是必不可少的。
