Steigemann Patrick, Gerlich Daniel W
Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstr. 18, CH-8093 Zurich, Switzerland.
Trends Cell Biol. 2009 Nov;19(11):606-16. doi: 10.1016/j.tcb.2009.07.008. Epub 2009 Sep 3.
The intercellular canal containing the midbody is one of the most prominent structures in dividing animal cells, yet its function in the completion of cytokinesis by abscission remains largely unknown. This is because of its small size, which makes it difficult to investigate the cytoskeletal and membrane dynamics underlying abscission by standard light microscopy. The advent of new fluorescent probes and imaging technologies, along with sophisticated perturbation tools, provides new possibilities to elucidate the molecular control of this essential cell biological process. Here we discuss the control of midbody assembly and current models for the mechanism of abscission in animal cells. We highlight new methodologies that will facilitate testing and refining of these models.
包含中间体的细胞间通道是动物细胞分裂过程中最显著的结构之一，然而其在通过缢裂完成胞质分裂中的功能仍 largely unknown。这是因为其尺寸小，使得难以通过标准光学显微镜研究缢裂背后的细胞骨架和膜动力学。新荧光探针和成像技术的出现，以及先进的扰动工具，为阐明这一基本细胞生物学过程的分子控制提供了新的可能性。在这里，我们讨论中间体组装的控制以及动物细胞中缢裂机制的当前模型。我们强调将有助于测试和完善这些模型的新方法。
