卵内注射胃饥饿素对新孵化雏鸡血清丙二醛水平的影响。
Effect of in ovo ghrelin administration on serum malondialdehyde level in newly-hatched chickens.
作者信息
Lotfi Alireza, Shahryar Habib Aghdam, Ebrahimnezhad Yahya, Shayegh Jalal
机构信息
Department of Animal Science, Shabestar Branch, Islamic Azad University, 53815-159 Shabestar, Iran.
出版信息
Asian Pac J Trop Biomed. 2012 Jan;2(1):47-9. doi: 10.1016/S2221-1691(11)60188-3.
OBJECTIVE
To investigate effects of in ovo ghrelin administration on serum malondialdehyde (MDA) level in newly-hatched chickens.
METHODS
Fertilized eggs were divided into 7 groups: group T1 as control (without injection), group T2 (in ovo injected with 50 ng/egg ghrelin on day 5), group T3 (in ovo injected with 100 ng/egg ghrelin on day 5), group T4 (in ovo injected with 50 ng/egg ghrelin on day 10), group T5 (in ovo injected with 100 ng/egg ghrelin on day 10), group T6 (in ovo injected with solvent: 1% acetic acid, without ghrelin on day 5) and group T7 (in ovo injected with solvent without ghrelin on day 10). After hatching, serum MDA concentrations were determined.
RESULTS
Ghrelin administrated groups (T2, T3, T4 and T5) had lower serum MDA level in comparison with control group (T1) or solvent injected groups (T6 and T7). T2 and T3 (ghrelin injection on day 5) had significantly lower MDA concentrations (4.10 and 4.60 nmol/mL, respectively) in comparison with other groups. In T4 and T5, MDA levels were lower than T1, T6 and T7 (non-ghrelin administrated groups) (9.53 and 9.50 in comparison with 10.73, 10.03 and 10.13 nmol/mL) and were higher than T2 and T3.
CONCLUSIONS
It can be concluded that in ovo administration of ghrelin can have anti-oxidative protection and reduce serum MDA level. Ghrelin administration on day 5 of incubation is more efficient.
目的
研究胚内注射胃饥饿素对新孵化雏鸡血清丙二醛（MDA）水平的影响。
方法
将受精蛋分为7组：T1组为对照组（不注射），T2组（在第5天胚内注射50 ng/蛋胃饥饿素），T3组（在第5天胚内注射100 ng/蛋胃饥饿素），T4组（在第10天胚内注射50 ng/蛋胃饥饿素），T5组（在第10天胚内注射100 ng/蛋胃饥饿素），T6组（在第5天胚内注射溶剂：1%乙酸，不注射胃饥饿素）和T7组（在第10天胚内注射无胃饥饿素的溶剂）。孵化后，测定血清MDA浓度。
结果
与对照组（T1）或注射溶剂组（T6和T7）相比，胃饥饿素给药组（T2、T3、T4和T5）的血清MDA水平较低。与其他组相比，T2和T3组（第5天注射胃饥饿素）的MDA浓度显著较低（分别为4.10和4.60 nmol/mL）。在T4和T5组中，MDA水平低于T1、T6和T7组（未给予胃饥饿素的组）（分别为9.53和9.50，而T1、T6和T7组分别为10.73、10.03和10.13 nmol/mL），且高于T2和T3组。
结论
可以得出结论，胚内注射胃饥饿素可具有抗氧化保护作用并降低血清MDA水平。在孵化第5天给予胃饥饿素更有效。
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