Khaligh F, Hassanabadi A, Nassiri-Moghaddam H, Golian A, Kalidari G-A
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
J Anim Physiol Anim Nutr (Berl). 2018 Feb;102(1):e413-e420. doi: 10.1111/jpn.12760. Epub 2017 Jun 21.
An experiment was conducted to evaluate the effects of in ovo injection of chrysin, quercetin and ascorbic acid on hatchability, somatic attributes, hepatic antioxidant status and early post-hatch growth performance of broiler chicks. Four hundred and eighty embryonated broiler breeder eggs containing live 18-day-old embryos were divided into six groups of 80 eggs each. One group remained intact and served as a control group (i), whereas the other five groups were injected with the prepared injection solutions as follows: (ii) 0.05 ml distilled water; (iii) 0.05 ml distilled water containing 6 mg ascorbic acid; (iv) 0.05 ml dimethyl sulfoxide (DMSO); (v) 0.05 ml DMSO containing 4.5 mg quercetin; and (vi) 0.05 ml DMSO containing 4.5 mg chrysin. The hatchability rate, hatching weight, residual yolk sac weight, yolk sac-free body weight, liver weight, hepatic glutathione peroxidase and total superoxide dismutase activities, as well as malondialdehyde concentrations, were not affected by the injected solutions. There were no differences between chicks hatched from the control and in ovo injected eggs in weight gain, feed intake and feed conversion ratio from 0 to 11 days of age. However, the specific contrast performed between the in ovo injected groups and intact eggs revealed that in ovo injection significantly increased hatchability rate (p = .0493). This finding also implies that our injection procedure was harmless. In conclusion, the intra-egg injection of chrysin, quercetin or ascorbic acid at the injection rates used in this study did not have a significant effect on hatchability, somatic characteristics, early growth performance and hepatic antioxidant status of broiler chicks. However, the overall hatchability was higher in the in ovo injected eggs as compared to non-injected ones. These findings also confirmed the harmlessness of the procedure developed for in ovo injection in this study.
进行了一项实验，以评估在鸡胚内注射白杨素、槲皮素和抗坏血酸对肉仔鸡孵化率、身体特征、肝脏抗氧化状态及出壳后早期生长性能的影响。480枚含有18日龄活胚的肉种鸡胚胎蛋被分为6组，每组80枚。一组保持完整作为对照组（i），而其他五组分别注射以下配制好的注射液：（ii）0.05毫升蒸馏水；（iii）含6毫克抗坏血酸的0.05毫升蒸馏水；（iv）0.05毫升二甲基亚砜（DMSO）；（v）含4.5毫克槲皮素的0.05毫升DMSO；（vi）含4.5毫克白杨素的0.05毫升DMSO。注射溶液对孵化率、出壳重、残留卵黄囊重、无卵黄囊体重、肝脏重量、肝脏谷胱甘肽过氧化物酶和总超氧化物歧化酶活性以及丙二醛浓度均无影响。0至11日龄时，对照组和经胚内注射的种蛋孵化出的雏鸡在体重增加、采食量和饲料转化率方面没有差异。然而，对胚内注射组和完整种蛋之间进行的特定对比显示，胚内注射显著提高了孵化率（p = 0.0493）。这一发现也表明我们的注射程序是无害的。总之，本研究中使用的注射剂量下，在鸡蛋内注射白杨素、槲皮素或抗坏血酸对肉仔鸡的孵化率、身体特征、早期生长性能和肝脏抗氧化状态没有显著影响。然而，与未注射的鸡蛋相比，经胚内注射的鸡蛋总体孵化率更高。这些发现也证实了本研究中开发的胚内注射程序是无害的。
