吡哆醛化血红蛋白聚氧乙烯共轭物和无基质血红蛋白对离体大鼠心脏心功能的影响。

Effects of pyridoxalated hemoglobin polyoxyethylene conjugate and stroma-free hemoglobin on cardiac function in isolated rat heart.

作者信息

Yamakawa T, Miyauchi Y, Nishi K

机构信息

Department of Pharmacology, Kumamoto University Medical School, Japan.

出版信息

Artif Organs. 1990 Jun;14(3):208-17. doi: 10.1111/j.1525-1594.1990.tb02959.x.

DOI:10.1111/j.1525-1594.1990.tb02959.x

PMID:2357147

Abstract

Effects of a newly introduced modified hemoglobin, pyridoxalated hemoglobin polyoxyethylene conjugate (PHP), and stroma-free hemoglobin (SFH) on cardiac rhythm and contractility were examined in isolated rat hearts. Hearts were perfused with HEPES-buffered Krebs solution containing 6% PHP, 6% SFH, or 6% hydroxyethylstarch (HES). Arrhythmia was not observed in PHP-perfused hearts, but marked sinus bradycardia occurred in SFH-perfused hearts. Ventricular contractility assessed by left ventricular pressure and pressure-volume relationship in both PHP and SFH-perfused hearts were well maintained during perfusion. There was no significant difference to the coronary flow resistance between hearts perfused with PHP and HES solutions, indicating that PHP has little vasoactive effect. The flow resistance in SFH-perfused hearts decreased significantly when compared with other groups. Results demonstrate that PHP solution can preserve cardiac function and have no toxic effects in isolated rat hearts.

摘要

在离体大鼠心脏中研究了新引入的修饰血红蛋白——吡哆醛化血红蛋白聚氧乙烯共轭物（PHP）和无基质血红蛋白（SFH）对心律和收缩性的影响。用含有6% PHP、6% SFH或6%羟乙基淀粉（HES）的HEPES缓冲Krebs溶液灌注心脏。在灌注PHP的心脏中未观察到心律失常，但在灌注SFH的心脏中出现了明显的窦性心动过缓。在灌注过程中，通过左心室压力和压力-容积关系评估的PHP和SFH灌注心脏的心室收缩性均得到良好维持。灌注PHP和HES溶液的心脏之间的冠状动脉血流阻力没有显著差异，表明PHP几乎没有血管活性作用。与其他组相比，SFH灌注心脏的血流阻力显著降低。结果表明，PHP溶液可以保留心脏功能，并且在离体大鼠心脏中没有毒性作用。