Nishi K, Ueda S, Sato D, Nishi K
Department of Pharmacology, Kumamoto University School of Medicine, Japan.
Artif Organs. 1994 Jun;18(6):429-38. doi: 10.1111/j.1525-1594.1994.tb02229.x.
The effects of pyridoxalated hemoglobin polyoxyethylene conjugate (PHP) and stroma-free hemoglobin (SFH) on vascular responsiveness to various vasoactive substances were examined in isolated perfused rat kidneys. The kidneys isolated from rats were perfused with 6% PHP, 6% SFH, and 6% hydroxyethylstarch (HES) solution at a constant flow rate. Vascular responsiveness to acetylcholine (ACh), nitroglycerin (NG), norepinephrine (NE), and angiotensin-II (ANG-II) was examined by measuring the perfusion pressure (PP). Effects of inhibition of endothelium-derived relaxing factor (EDRF) by NG-monomethyl L-arginine (L-NMMA) on NE-induced and ANG-II-induced renal vascular responses were examined. ACh and NG induced a dose-dependent decrease in perfusion pressure (PP) in all groups. NE and ANG-II induced an increase in PP in all groups, but NE-induced and ANG-II-induced responses in the PHP-perfused and SFH-perfused groups were significantly larger than those in the HES-perfused group. L-NMMA did not alter vascular responsiveness to NE and ANG-II. These results indicate that PHP and SFH do not inhibit EDRF induced by ACh, but hemoglobin moiety per se does augment the vascular responsiveness to NE and ANG-II in the isolated perfused rat kidney.
在离体灌注大鼠肾脏中，研究了吡哆醛化血红蛋白聚氧乙烯共轭物（PHP）和无基质血红蛋白（SFH）对血管对各种血管活性物质反应性的影响。将从大鼠分离的肾脏以恒定流速用6%的PHP、6%的SFH和6%的羟乙基淀粉（HES）溶液进行灌注。通过测量灌注压力（PP）来检测血管对乙酰胆碱（ACh）、硝酸甘油（NG）、去甲肾上腺素（NE）和血管紧张素II（ANG-II）的反应性。研究了NG-单甲基L-精氨酸（L-NMMA）抑制内皮源性舒张因子（EDRF）对NE诱导和ANG-II诱导的肾血管反应的影响。ACh和NG在所有组中均引起灌注压力（PP）呈剂量依赖性降低。NE和ANG-II在所有组中均引起PP升高，但PHP灌注组和SFH灌注组中NE诱导和ANG-II诱导的反应明显大于HES灌注组。L-NMMA未改变血管对NE和ANG-II的反应性。这些结果表明，PHP和SFH不抑制ACh诱导的EDRF，但血红蛋白部分本身确实增强了离体灌注大鼠肾脏中血管对NE和ANG-II的反应性。
