Jang Ja-Young, Cai Jingmei, Kim Jihyun, Kyung Jangbeen, Kim Dajeong, Choi Ehn-Kyoung, Kim Youngeun, Kim Kwang-Sei, Park Dongsun, Kang Hyun-Gu, Kim Yun-Bae
College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Lab Anim Res. 2013 Mar;29(1):7-11. doi: 10.5625/lar.2013.29.1.7. Epub 2013 Mar 25.
According to a high anti-osteoporotic efficacy of Sigma Anti-bonding Molecule Calcium Carbonate (SAC), repeated-dose toxicities of SAC were investigated to assess its feasibility as drug or functional food ingredient. Male ICR mice were given drinking water containing 0.006, 0.02 or 0.06% SAC for 4 weeks. SAC feeding decreased the body weights and feed and water consumptions of mice in a dose-dependent manner, especially, leading to severe emaciation and 70% death in 3 weeks in the high-dose (0.06%) group. Not only kidney and heart weights, but also the levels of blood urea nitrogen, creatinine, aspartate transaminase, and creatine phospokinase significantly increased after SAC administration, indicative of nephrotoxicity and cardiotoxicity. Such renal and cardiac toxicities were also confirmed by microscopic findings, exhibiting renal crystals and cardiac fibrosis, which may be due to the insoluble crystal formation and calcium overload, respectively. In conclusion, it is suggested that no observed adverse effect level of SAC is lower than 0.006% in mice, and that a long-term intake may cause serious adverse effects on renal and cardiac functions.
鉴于西格玛反键分子碳酸钙（SAC）具有较高的抗骨质疏松功效，对SAC进行了重复给药毒性研究，以评估其作为药物或功能性食品成分的可行性。将雄性ICR小鼠给予含0.006%、0.02%或0.06% SAC的饮用水，持续4周。给予SAC后，小鼠体重、饲料和水消耗量呈剂量依赖性下降，尤其是高剂量（0.06%）组在3周内导致严重消瘦和70%死亡。给予SAC后，不仅肾脏和心脏重量增加，而且血尿素氮、肌酐、天冬氨酸转氨酶和肌酸磷酸激酶水平显著升高，表明存在肾毒性和心脏毒性。肾脏和心脏的这些毒性也通过显微镜检查结果得到证实，分别表现为肾结晶和心脏纤维化，这可能分别是由于不溶性晶体形成和钙超载所致。总之，提示在小鼠中SAC的未观察到不良反应水平低于0.006%，长期摄入可能对肾脏和心脏功能造成严重不良反应。
