Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, New Delhi 110062, India.
Toxicol Mech Methods. 2011 Jan;21(1):41-7. doi: 10.3109/15376516.2010.529188. Epub 2010 Nov 10.
Doxorubicin is one of the most active cytotoxic agents in current use. The clinical usefulness of the doxorubicin has been precluded by its marked cardiotoxicity. The aim of the present study was to investigate the effect of Khamira Abresham Hakim Arshadwala, a well known unani cardiac tonic formulation, pre-treatment on doxorubicin-induced cardiotoxicity, and nephrotoxicity in rats. Twenty-four Wistar albino rats, divided into four groups, were used. Khamira Abresham Hakim Arshadwala was administered daily, for 7 days, and a single dose of doxorubicin (10 mg/kg, i.v.) on day 5. After 48 h of doxorubicin injection, animals were sacrificed. Lactate dehydrogenase (LDH), aspartate transaminase (AST), blood urea nitrogen (BUN), and creatinine were estimated in the serum. Heart specimens were used for biochemical estimations of lipid peroxides (MDA), reduced glutathione (GSH), catalase, and for microscopic examination of histopathological changes. Doxorubicin showed cardiotoxicity and nephrotoxicity, as evidenced by elevated activities of AST, LDH, BUN, creatinine, and MDA, depletion in GSH level, and catalase activity. Histopathological studies showed disruption of cardiac tissues in doxorubicin groups. Khamira Abresham Hakim Arshadwala pre-treatment showed a protective effect against the enzymatic changes in serum as well as cardiac and kidney tissue damage, significantly. The present findings suggest that Khamira Abresham Hakim Arshadwala significantly (p < 0.01) improved the state of markers for cardiac and kidney damage investigated in this model of doxorubicin-induced experimental cardiotoxicity and nephrotoxicity; indicating its potential in limiting doxorubicin toxicity.
多柔比星是目前使用的最有效的细胞毒性药物之一。由于其明显的心脏毒性，多柔比星的临床应用受到限制。本研究旨在探讨一种著名的顺势心脏滋补剂 Khamira Abresham Hakim Arshadwala 预处理对多柔比星诱导的大鼠心脏毒性和肾毒性的影响。将 24 只 Wistar 白化大鼠分为四组。Khamira Abresham Hakim Arshadwala 每天给药，共 7 天，第 5 天给予单次多柔比星（10mg/kg，iv）。多柔比星注射后 48 小时，处死动物。血清中测定乳酸脱氢酶（LDH）、天门冬氨酸转氨酶（AST）、血尿素氮（BUN）和肌酐。心脏标本用于测定脂质过氧化物（MDA）、还原型谷胱甘肽（GSH）、过氧化氢酶的生化估计值，并进行组织病理学变化的显微镜检查。多柔比星表现出心脏毒性和肾毒性，这表现在 AST、LDH、BUN、肌酐和 MDA 活性升高，GSH 水平和过氧化氢酶活性降低。组织病理学研究表明，多柔比星组心脏组织受损。Khamira Abresham Hakim Arshadwala 预处理对血清酶以及心脏和肾脏组织损伤的变化具有保护作用，具有显著的统计学意义（p<0.01）。本研究结果表明，Khamira Abresham Hakim Arshadwala 显著（p<0.01）改善了多柔比星诱导的实验性心脏毒性和肾毒性模型中心脏和肾脏损伤标志物的状态；表明其具有限制多柔比星毒性的潜力。
