Hargrove J L, Hulsey M G, Schmidt F H, Beale E G
Department of Foods and Nutrition, University of Georgia, Athens, 30602.
Biotechniques. 1990 Jun;8(6):654-9.
Enzyme induction may be modeled on the basis of four, quantifiable processes that control the rates at which specific gene products accumulate and decay. These processes include synthesis of functional mRNA, translation and degradation of mRNA, and degradation of the protein product. We present a simple computer program that permits mathematical simulation of gene expression on the basis of experimentally determined rates of synthesis and degradation. The program was implemented as a spreadsheet using Microsoft Excel for Macintosh and MS-DOS operating systems and also was adapted for HyperCard on the Macintosh. It contains a formula to account for growth of tissue or cell populations. The program predicts amounts of individual mRNAs and proteins (or enzyme activities) in cells as a function of time after a stimulus alters their rates of synthesis or degradation.
酶诱导可以基于四个可量化的过程进行建模，这些过程控制着特定基因产物积累和降解的速率。这些过程包括功能性mRNA的合成、mRNA的翻译和降解以及蛋白质产物的降解。我们提出了一个简单的计算机程序，该程序可以根据实验确定的合成和降解速率对基因表达进行数学模拟。该程序使用适用于Macintosh和MS-DOS操作系统的Microsoft Excel作为电子表格来实现，并且也适用于Macintosh上的HyperCard。它包含一个用于计算组织或细胞群体生长的公式。该程序可预测在刺激改变其合成或降解速率后，细胞中单个mRNA和蛋白质（或酶活性）的量随时间的变化。
