Stahl Jessica, Mielke Stefanie, Pankow Wolf-Rüdiger, Kietzmann Manfred
BMC Vet Res. 2013 Apr 10;9:70. doi: 10.1186/1746-6148-9-70.
An in-vitro setup was established in order to determine a) the diffusion activities of eight otic preparations (Aurizon®, Eas Otic®, Epi Otic®, Otifree®, Otomax®, Panolog®, Posatex®, Surolan®) through synthetic cerumen, and b) the ceruminolytic capacity and impregnation effects of these products. The main lipid classes of canine cerumen produced with moderate, non-purulent otitis externa were determined by thin layer chromatography and were subsequently used to produce a standardised synthetic cerumen (SCC). SCC was filled into capillary tubes, all of which were loaded with six commercially available multipurpose otic medications and two ear cleaners, each mixed with two markers in two experimental setups. These two marker compounds (Oil red O and marbofloxacin) were chosen, since they exhibit different physicochemical drug characteristics by which it is possible to determine and verify the diffusion activity of different types of liquids (i.e. the otic preparations). A synthetic cerumen described in the literature (JSL) was also used for comparison as its lipid composition was different to SCC. The diffusion activities of the otic preparations through both types of synthetic cerumen were studied over 24 hours. A second in-vitro experiment determined both the ceruminolytic activity and impregnation effect of the otic preparations by comparing the weight loss or weight gain after repeated incubation of JSL.
Canine cerumen is mainly composed of triglycerides, sterol esters, fatty acid esters and squalene. The diffusion experiments showed a high diffusion efficacy along with a high impregnation effect for one test product. All the other products exhibited a lower diffusion activity with a mild to moderate impregnation effect. A mild ceruminolytic activity was observed for the two ear cleaners but not for any of the otic medications.
The present study demonstrates that there are significant differences in the diffusion characteristics and ceruminolytic properties of the eight tested otic preparations.
建立了一种体外实验装置，以确定：a）八种耳部制剂（Aurizon®、Eas Otic®、Epi Otic®、Otifree®、Otomax®、Panolog®、Posatex®、Surolan®）通过合成耵聍的扩散活性；b）这些产品的耵聍溶解能力和浸渍效果。通过薄层色谱法测定了中度非化脓性外耳道炎犬耵聍的主要脂质类别，随后用于制备标准化合成耵聍（SCC）。将SCC填充到毛细管中，在两个实验装置中，所有毛细管都装载了六种市售的多用途耳部药物和两种耳部清洁剂，每种都与两种标记物混合。选择这两种标记化合物（油红O和马波沙星）是因为它们具有不同的物理化学药物特性，通过这些特性可以确定和验证不同类型液体（即耳部制剂）的扩散活性。文献中描述的一种合成耵聍（JSL）也用于比较，因为其脂质组成与SCC不同。研究了耳部制剂在24小时内通过两种类型合成耵聍的扩散活性。第二个体外实验通过比较JSL反复孵育后的重量损失或重量增加，确定了耳部制剂的耵聍溶解活性和浸渍效果。
犬耵聍主要由甘油三酯、甾醇酯、脂肪酸酯和角鲨烯组成。扩散实验表明，一种测试产品具有高扩散效率和高浸渍效果。所有其他产品表现出较低的扩散活性，浸渍效果为轻度至中度。两种耳部清洁剂观察到轻度耵聍溶解活性，但耳部药物均未观察到。
本研究表明，八种受试耳部制剂的扩散特性和耵聍溶解特性存在显著差异。
Zotero 插件
