Levi-Montalcini R, Aloe L, Mugnaini E, Oesch F, Thoenen H
Proc Natl Acad Sci U S A. 1975 Feb;72(2):595-9. doi: 10.1073/pnas.72.2.595.
Concomitant daily treatment of newborn rats for a 2-week to 1-month period with 10 mug/g of body weight of nerve growth factor and 100 mug/g of body weight of 6-hydroxydopamine produces in the cell bodies of adrenergic neurons the characteristic effects of the growth factor but in the nerve terminals the characteristic effects of 6-hydroxydopamine. The dual opposite effects result in a striking volume increase of sympathetic ganglia which far exceeds that produced by nerve growth factor alone. The selective induction of tyrosine hydroxylase [L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] in these chemically axotomized adrenergic neurons is even more pronounced than that produced by nerve growth factor alone in intact neurons.
新生大鼠每日同时接受为期2周～1个月的治疗，给予体重10μg/g的神经生长因子和体重100μg/g的6-羟基多巴胺，结果显示，在肾上腺素能神经元的细胞体中产生了生长因子的特征性效应，而在神经末梢则产生了6-羟基多巴胺的特征性效应。这两种相反的效应导致交感神经节显著增大，其增大程度远远超过单独使用神经生长因子所产生的效果。在这些化学性轴突切断的肾上腺素能神经元中，酪氨酸羟化酶[L-酪氨酸，四氢蝶啶：氧氧化还原酶(3-羟化)，EC 1.14.16.2]的选择性诱导甚至比完整神经元中单独使用神经生长因子所产生的诱导作用更为明显。
