Department of Biomaterials, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Macromol Biosci. 2013 Jun;13(6):777-88. doi: 10.1002/mabi.201200474. Epub 2013 Apr 10.
The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10 mg ml(-1) ) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10 mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (≈5-20 µm) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.
酶碱性磷酸酶（ALP）以不同浓度（即 0、2.5 和 10mg/ml）添加到聚（聚乙二醇）富马酸酯（OPF）水凝胶中。支架要么在 10mM 甘油磷酸钙（Ca-GP）溶液中孵育 2 周，要么在大鼠皮下模型中植入 4 周。傅里叶变换红外（FTIR）光谱、X 射线衍射（XRD）、扫描电子显微镜（SEM）、能谱（EDS）和茜素红染色显示，在体外仅在含有 ALP 的水凝胶中具有很强的形成矿物质的能力。此外，钙含量随 ALP 浓度的增加而增加。同样，只有含有 ALP 的水凝胶在体内诱导矿化。具体而言，使用 Von Kossa 和茜素红染色，在靠近真皮/支架界面的水凝胶外围观察到小（≈5-20μm）矿物质沉积物。
