通过加入碱性磷酸酶对水凝胶进行酶促矿化用于骨组织工程。
Enzymatic mineralization of hydrogels for bone tissue engineering by incorporation of alkaline phosphatase.
机构信息
Department of Biomaterials, Radboud University Medical Center Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
出版信息
Macromol Biosci. 2012 Aug;12(8):1077-89. doi: 10.1002/mabi.201100501. Epub 2012 May 30.
Abstract
Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG > collagen > OPF. The mineral:polymer ratio decreases in the order collagen > cPEG > OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.
摘要
碱性磷酸酶（ALP）是一种参与骨矿化的酶，被掺入三种水凝胶生物材料中，以诱导其矿化形成磷酸钙（CaP）。这些材料包括胶原蛋白 I 型、一种由带有儿茶酚基团的聚乙二醇（PEG）取代的贻贝类蛋白胶粘剂 cPEG，以及聚乙二醇/富马酸共聚物 OPF。在 Ca-GP 溶液中孵育后，通过傅里叶变换红外光谱（FTIR）、能谱分析（EDS）、扫描电子显微镜（SEM）、X 射线衍射（XRD）、选区电子衍射（SAED）、电感耦合等离子体发射光谱（ICP-OES）和 von Kossa 染色证实了 CaP 的形成。形成的矿物量按 cPEG > 胶原蛋白 > OPF 的顺序减少。矿物/聚合物的比例按胶原蛋白 > cPEG > OPF 的顺序减少。矿化作用增加了杨氏模量，cPEG 的增加最为显著。这种酶矿化水凝胶/CaP 复合材料可能在骨再生材料中有应用前景。
相似文献
1
Enzymatic mineralization of hydrogels for bone tissue engineering by incorporation of alkaline phosphatase.通过加入碱性磷酸酶对水凝胶进行酶促矿化用于骨组织工程。
Macromol Biosci. 2012 Aug;12(8):1077-89. doi: 10.1002/mabi.201100501. Epub 2012 May 30.
2
Enzymatic mineralization of gellan gum hydrogel for bone tissue-engineering applications and its enhancement by polydopamine.用于骨组织工程应用的结冷胶水凝胶的酶促矿化及其通过聚多巴胺的增强作用。
J Tissue Eng Regen Med. 2014 Nov;8(11):906-18. doi: 10.1002/term.1616. Epub 2012 Oct 5.
3
Generation of composites for bone tissue-engineering applications consisting of gellan gum hydrogels mineralized with calcium and magnesium phosphate phases by enzymatic means.通过酶法制备由钙和镁磷酸盐相矿化的结冷胶水凝胶组成的用于骨组织工程应用的复合材料。
J Tissue Eng Regen Med. 2016 Nov;10(11):938-954. doi: 10.1002/term.1875. Epub 2014 Feb 21.
4
Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase.碱性磷酸酶加速壳聚糖水凝胶的胶凝和矿化。
Int J Biol Macromol. 2013 May;56:122-32. doi: 10.1016/j.ijbiomac.2013.02.002. Epub 2013 Feb 8.
5
In vitro and in vivo enzyme-mediated biomineralization of oligo(poly(ethylene glycol) fumarate hydrogels.体外和体内酶介导的聚（聚乙二醇）富马酸水凝胶的生物矿化。
Macromol Biosci. 2013 Jun;13(6):777-88. doi: 10.1002/mabi.201200474. Epub 2013 Apr 10.
6
Amorphous polyphosphate-hydroxyapatite: A morphogenetically active substrate for bone-related SaOS-2 cells in vitro.无定形多聚磷酸盐-羟磷灰石：一种体外骨相关 SaOS-2 细胞形态发生活性基质。
Acta Biomater. 2016 Feb;31:358-367. doi: 10.1016/j.actbio.2015.11.060. Epub 2015 Nov 30.
7
Injectable self-gelling composites for bone tissue engineering based on gellan gum hydrogel enriched with different bioglasses.基于富含不同生物玻璃的结冷胶水凝胶的用于骨组织工程的可注射自凝胶复合材料。
Biomed Mater. 2014 Aug;9(4):045014. doi: 10.1088/1748-6041/9/4/045014. Epub 2014 Jul 25.
8
Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol(®) to endow antibacterial properties and promote mineralization.用富含间苯三酚单宁的海带提取物Seanol(®)对酶促矿化的结冷胶水凝胶进行富集，以赋予其抗菌性能并促进矿化。
Biomed Mater. 2016 Aug 10;11(4):045015. doi: 10.1088/1748-6041/11/4/045015.
9
In vivo bone and soft tissue response to injectable, biodegradable oligo(poly(ethylene glycol) fumarate) hydrogels.可注射、可生物降解的聚（乙二醇富马酸酯）寡聚物水凝胶在体内的骨与软组织反应
Biomaterials. 2003 Aug;24(19):3201-11. doi: 10.1016/s0142-9612(03)00168-6.
10
Synergistic intrafibrillar/extrafibrillar mineralization of collagen scaffolds based on a biomimetic strategy to promote the regeneration of bone defects.基于仿生策略的胶原蛋白支架协同纤维内/纤维外矿化以促进骨缺损再生
Int J Nanomedicine. 2016 May 12;11:2053-67. doi: 10.2147/IJN.S102844. eCollection 2016.
引用本文的文献
1
Review of collagen type I-based hydrogels: focus on composition-structure-properties relationships.基于I型胶原蛋白的水凝胶综述：聚焦组成-结构-性能关系
NPJ Biomed Innov. 2025;2(1):16. doi: 10.1038/s44385-025-00018-w. Epub 2025 May 3.
2
Understanding Matrix Stiffness in Vinyl Polymer Hydrogels: Implications in Bone Tissue Engineering.理解乙烯基聚合物水凝胶中的基质刚度：对骨组织工程的启示
ACS Omega. 2024 Mar 25;9(16):17891-17902. doi: 10.1021/acsomega.3c08877. eCollection 2024 Apr 23.
3
Enzyme-Triggered Crosslinked Hybrid Hydrogels for Bone Tissue Engineering.用于骨组织工程的酶触发交联混合水凝胶
Materials (Basel). 2022 Sep 14;15(18):6383. doi: 10.3390/ma15186383.
4
Cryogel Scaffold-Mediated Delivery of Adipose-Derived Stem Cells Promotes Healing in Murine Model of Atrophic Non-Union.冷冻凝胶支架介导的脂肪干细胞递送促进萎缩性骨不连小鼠模型的愈合。
Front Bioeng Biotechnol. 2022 May 5;10:851904. doi: 10.3389/fbioe.2022.851904. eCollection 2022.
5
Electron Beam-Treated Enzymatically Mineralized Gelatin Hydrogels for Bone Tissue Engineering.用于骨组织工程的电子束处理酶促矿化明胶水凝胶
J Funct Biomater. 2021 Oct 8;12(4):57. doi: 10.3390/jfb12040057.
6
Human Adipose-Derived Hydrogel Characterization Based on ASC Biocompatibility and Differentiation.基于脂肪干细胞生物相容性和分化的人脂肪源性水凝胶特性分析
Stem Cells Int. 2019 Dec 27;2019:9276398. doi: 10.1155/2019/9276398. eCollection 2019.
7
Modulation of proliferation and differentiation of gingiva‑derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair.浓缩生长因子对牙龈间充质干细胞增殖和分化的调节：在牙组织再生和修复的组织工程中的潜在意义。
Int J Mol Med. 2019 Jul;44(1):37-46. doi: 10.3892/ijmm.2019.4172. Epub 2019 Apr 24.
8
Magnesium Modifies the Structural Features of Enzymatically Mineralized Collagen Gels Affecting the Retraction Capabilities of Human Dermal Fibroblasts Embedded within This 3D System.镁改变酶促矿化胶原凝胶的结构特征，影响嵌入该三维系统的人真皮成纤维细胞的收缩能力。
Materials (Basel). 2016 Jun 15;9(6):477. doi: 10.3390/ma9060477.
9
Engineered HA hydrogel for stem cell transplantation in the brain: Biocompatibility data using a design of experiment approach.用于脑内干细胞移植的工程化透明质酸水凝胶：采用实验设计方法的生物相容性数据
Data Brief. 2016 Nov 24;10:202-209. doi: 10.1016/j.dib.2016.11.069. eCollection 2017 Feb.
10
Development of thermosensitive hydrogels of chitosan, sodium and magnesium glycerophosphate for bone regeneration applications.壳聚糖、甘油磷酸钠和镁的温敏水凝胶的开发及其在骨再生中的应用。
J Funct Biomater. 2015 Apr 9;6(2):192-203. doi: 10.3390/jfb6020192.
本文引用的文献
1
Enzyme Directed Templating of Artificial Bone Mineral.酶促人工骨矿物质模板化
Adv Mater. 2009 Jan 26;21(4):425-430. doi: 10.1002/adma.200802242.
2
Cell encapsulation using biopolymer gels for regenerative medicine.使用生物聚合物凝胶进行细胞封装用于再生医学。
Biotechnol Lett. 2010 Jun;32(6):733-42. doi: 10.1007/s10529-010-0221-0. Epub 2010 Feb 13.
3
The mechanism of mineralization and the role of alkaline phosphatase in health and disease.矿化机制以及碱性磷酸酶在健康与疾病中的作用。
J Nippon Med Sch. 2010 Feb;77(1):4-12. doi: 10.1272/jnms.77.4.
4
Injectable candidate sealants for fetal membrane repair: bonding and toxicity in vitro.可注射候选胎膜修复剂：体外黏合和毒性。
Am J Obstet Gynecol. 2010 Jan;202(1):85.e1-9. doi: 10.1016/j.ajog.2009.07.051.
5
Biological performance of mussel-inspired adhesive in extrahepatic islet transplantation.贻贝启发型黏附剂在外周胰岛移植中的生物学性能。
Biomaterials. 2010 Jan;31(3):420-7. doi: 10.1016/j.biomaterials.2009.09.062. Epub 2009 Oct 6.
6
Substrate stiffness affects early differentiation events in embryonic stem cells.基质硬度会影响胚胎干细胞的早期分化事件。
Eur Cell Mater. 2009 Sep 21;18:1-13; discussion 13-4. doi: 10.22203/ecm.v018a01.
7
Sodium citrate as an effective dispersant for the synthesis of inorganic-organic composites with a nanodispersed mineral phase.柠檬酸钠作为一种有效的分散剂，用于合成具有纳米分散矿物相的无机-有机复合材料。
Acta Biomater. 2010 Mar;6(3):836-44. doi: 10.1016/j.actbio.2009.09.005. Epub 2009 Sep 12.
8
The effect of alkaline phosphatase coated onto titanium alloys on bone responses in rats.涂覆在钛合金上的碱性磷酸酶对大鼠骨反应的影响。
Biomaterials. 2009 Nov;30(32):6407-17. doi: 10.1016/j.biomaterials.2009.08.001. Epub 2009 Aug 31.
9
Direct transformation from amorphous to crystalline calcium phosphate facilitated by motif-programmed artificial proteins.基序编程人工蛋白促进无定形磷酸钙向结晶磷酸钙的直接转变。
Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):16866-70. doi: 10.1073/pnas.0804277105. Epub 2008 Oct 28.
10
Amorphous calcium phosphate is a major component of the forming fin bones of zebrafish: Indications for an amorphous precursor phase.无定形磷酸钙是斑马鱼鳍骨形成过程中的主要成分：无定形前体相的指征。
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12748-53. doi: 10.1073/pnas.0803354105. Epub 2008 Aug 27.
Zotero 插件