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通过加入碱性磷酸酶对水凝胶进行酶促矿化用于骨组织工程。

Enzymatic mineralization of hydrogels for bone tissue engineering by incorporation of alkaline phosphatase.

机构信息

Department of Biomaterials, Radboud University Medical Center Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.

出版信息

Macromol Biosci. 2012 Aug;12(8):1077-89. doi: 10.1002/mabi.201100501. Epub 2012 May 30.

DOI:10.1002/mabi.201100501
PMID:22648976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3690824/
Abstract

Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG > collagen > OPF. The mineral:polymer ratio decreases in the order collagen > cPEG > OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.

摘要

碱性磷酸酶（ALP）是一种参与骨矿化的酶，被掺入三种水凝胶生物材料中，以诱导其矿化形成磷酸钙（CaP）。这些材料包括胶原蛋白 I 型、一种由带有儿茶酚基团的聚乙二醇（PEG）取代的贻贝类蛋白胶粘剂 cPEG，以及聚乙二醇/富马酸共聚物 OPF。在 Ca-GP 溶液中孵育后，通过傅里叶变换红外光谱（FTIR）、能谱分析（EDS）、扫描电子显微镜（SEM）、X 射线衍射（XRD）、选区电子衍射（SAED）、电感耦合等离子体发射光谱（ICP-OES）和 von Kossa 染色证实了 CaP 的形成。形成的矿物量按 cPEG > 胶原蛋白 > OPF 的顺序减少。矿物/聚合物的比例按胶原蛋白 > cPEG > OPF 的顺序减少。矿化作用增加了杨氏模量，cPEG 的增加最为显著。这种酶矿化水凝胶/CaP 复合材料可能在骨再生材料中有应用前景。

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