Courtin F, Gavaret J M, Toru-Delbauffe D, Pierre M
Unité de Recherche sur la Glande Thyroïde et la Régulation Hormonale, Institut National de la Santé et de la Recherche Médicale, Kremlin-Bicêtre, France.
Brain Res Dev Brain Res. 1990 May 1;53(2):237-42. doi: 10.1016/0165-3806(90)90012-n.
Acidic fibroblast growth factor (aFGF) induced a large increase in the type II 5'-deiodinase (5'D) activity in astroglial cells. This required a time lag of about 4 h. Half-maximal stimulation was obtained with about 7 ng/ml aFGF. This factor at 20 ng/ml induced several times more 5'D activity than did 20 ng/ml basic fibroblast growth factor (bFGF) after 8 h incubation. aFGF (20 ng/ml) produced a 10-50-fold increase in 5'D activity after 24 h, whereas the effect of 20 ng/ml bFGF had disappeared after 24 h. Heparin (17 micrograms/ml) potentiated the 5'D response to natural and recombinant aFGF. Glucocorticoids amplified the aFGF-induction of 5'D activity. This is the first demonstration in astroglial cells that a growth factor can regulate the 5'D activity.
酸性成纤维细胞生长因子（aFGF）可使星形胶质细胞中II型5'-脱碘酶（5'D）的活性大幅增加。这需要约4小时的时间延迟。约7 ng/ml的aFGF可获得半数最大刺激效果。在孵育8小时后，20 ng/ml的该因子诱导产生的5'D活性比20 ng/ml碱性成纤维细胞生长因子（bFGF）诱导产生的活性高几倍。24小时后，20 ng/ml的aFGF使5'D活性增加了10 - 50倍，而20 ng/ml的bFGF在24小时后的作用已消失。肝素（17微克/毫升）增强了5'D对天然和重组aFGF的反应。糖皮质激素放大了aFGF对5'D活性的诱导作用。这是首次在星形胶质细胞中证明生长因子可调节5'D活性。
