Australian Regenerative Medicine Institute-ARMI, Monash University, Clayton 3800, Victoria, Australia.
J Immunol Methods. 2013 Jul 31;393(1-2):74-80. doi: 10.1016/j.jim.2013.03.012. Epub 2013 Apr 9.
The adult mouse heart is comprised of a highly heterogeneous cell population. Isolation and effective cellular and molecular analysis of various cell types are critical for understanding cardiac development, homeostasis and disease. Moreover, strategies to isolate and analyse the complex inflammatory and tissue remodelling cell types that follow cardiac injury are particularly important for development of strategies to improve cardiac repair. Here we describe in detail how non-cardiomyocytes can be successfully isolated from the mouse heart. In addition, we describe how these isolation methods can be effectively coupled with flow cytometry, fluorescence activated cell sorting and/or magnetic-labelling to analyse and enrich cells for subsequent cellular or molecular analyses.
成年老鼠的心脏由高度异质的细胞群体组成。分离和有效分析各种细胞类型对于理解心脏发育、稳态和疾病至关重要。此外，分离和分析心脏损伤后复杂的炎症和组织重塑细胞类型的策略对于开发改善心脏修复的策略尤为重要。在这里，我们详细描述了如何从老鼠心脏中成功分离非心肌细胞。此外，我们还描述了如何将这些分离方法有效地与流式细胞术、荧光激活细胞分选和/或磁标记相结合，以分析和富集细胞，用于随后的细胞或分子分析。
