Ahmed Abdalla, Delgado-Olguin Paul
Translational Medicine, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.
Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Methods Mol Biol. 2018;1752:91-100. doi: 10.1007/978-1-4939-7714-7_9.
Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the isolation of differentiating mouse embryonic cardiac progenitors and cardiomyocytes at embryonic day (E) 9.5 and E13.5, respectively by FACS. Over 50,000 differentiating cardiac progenitors and 200,000 cardiomyocytes can be obtained in a single prep using the methods described.
分离高度纯化的胚胎心肌细胞群体能够在细胞水平上研究先天性心脏表型。荧光激活细胞分选（FACS）通常用于分离荧光标记的细胞。在此，我们描述了分别通过FACS在胚胎第9.5天（E9.5）和第13.5天（E13.5）分离分化中的小鼠胚胎心脏祖细胞和心肌细胞的方法。使用所述方法，单次制备可获得超过50,000个分化中的心脏祖细胞和200,000个心肌细胞。
