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非洲爪蟾U3小核仁RNA的体内破坏会影响核糖体RNA的加工。

In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.

作者信息

Savino R, Gerbi S A

机构信息

Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912.

出版信息

EMBO J. 1990 Jul;9(7):2299-308. doi: 10.1002/j.1460-2075.1990.tb07401.x.

DOI:10.1002/j.1460-2075.1990.tb07401.x
PMID:2357971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC551956/
Abstract

DNA oligonucleotide complementary to sequences in the 5' third of U3 snRNA were injected into Xenopus oocyte nuclei to disrupt endogenous U3 snRNA. The effect of this treatment on rRNA processing was examined. We found that some toads have a single rRNA processing pathway, whereas in other toads, two rRNA processing pathways can coexist in a single oocyte. U3 snRNA disruption in toads with the single rRNA processing pathway caused a reduction in 20S and '32S' pre-rRNA. In addition, in toads with two rRNA processing pathways, an increase in '36S' pre-rRNA of the second pathway is observed. This is the first in vivo demonstration that U3 snRNA plays a role in rRNA processing. Cleavage site #3 is at the boundary of ITS 1 and 5.8S and links all of the affected rRNA intermediates: 20S and '32S' are the products of site #3 cleavage in the first pathway and '36S' is the substrate for cleavage at site #3 in the second pathway. We postulate that U3 snRNP folds pre-rRNA into a conformation dictating correct cleavage at processing site #3.

摘要

将与U3小核仁RNA（snRNA）5'端三分之一序列互补的DNA寡核苷酸注入非洲爪蟾卵母细胞核中，以破坏内源性U3 snRNA。研究了这种处理对核糖体RNA（rRNA）加工的影响。我们发现，一些蟾蜍具有单一的rRNA加工途径，而在其他蟾蜍中，两种rRNA加工途径可以在单个卵母细胞中共存。在具有单一rRNA加工途径的蟾蜍中破坏U3 snRNA会导致20S和“32S”前体rRNA减少。此外，在具有两种rRNA加工途径的蟾蜍中，观察到第二种途径的“36S”前体rRNA增加。这是首次在体内证明U3 snRNA在rRNA加工中起作用。切割位点3位于内部转录间隔区1（ITS 1）和5.8S的边界，连接所有受影响的rRNA中间体：20S和“32S”是第一条途径中位点3切割的产物，“36S”是第二条途径中位点3切割的底物。我们推测，U3小核核糖核蛋白颗粒（snRNP）将前体rRNA折叠成一种构象，决定在加工位点3进行正确切割。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/83444c51a58b/emboj00234-0268-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/af9b6c8bf3c2/emboj00234-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/3f17098090c4/emboj00234-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/c55e76a5c4a8/emboj00234-0265-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/72342afaa573/emboj00234-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/fb86acf20f0b/emboj00234-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/a63236643377/emboj00234-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/5767df238b8b/emboj00234-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/83444c51a58b/emboj00234-0268-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/af9b6c8bf3c2/emboj00234-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/3f17098090c4/emboj00234-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/c55e76a5c4a8/emboj00234-0265-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/72342afaa573/emboj00234-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/fb86acf20f0b/emboj00234-0267-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/a63236643377/emboj00234-0267-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/5767df238b8b/emboj00234-0268-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/784c/551956/83444c51a58b/emboj00234-0268-b.jpg

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